TY - JOUR
T1 - Human CAR NK cells: A new non-viral method allowing high efficient transfection and strong tumor cell killing
AU - Ingegnere, Tiziano
AU - Mariotti, Francesca Romana
AU - Pelosi, Andrea
AU - Quintarelli, Concetta
AU - De Angelis, Biagio
AU - Tumino, Nicola
AU - Besi, Francesca
AU - Cantoni, Claudia
AU - Locatelli, Franco
AU - Vacca, Paola
AU - Moretta, Lorenzo
PY - 2019
Y1 - 2019
N2 - CAR-NK cells may represent a valuable tool, complementary to CART cells, in adoptive immunotherapy of leukemia and solid tumors. However, gene transfer to human NK cells is a challenging task, particularly with non-virus-based techniques. Here, we describe a new procedure allowing efficient electroporation-based transfection of plasmid DNA, including CAR and CCR7 genes, in resting or cytokine-expanded human NK cell populations and NK-92 cell line. This procedure may offer a suitable platform for a safe and effective use of CAR-NK cells in adoptive immunotherapy of cancer.
AB - CAR-NK cells may represent a valuable tool, complementary to CART cells, in adoptive immunotherapy of leukemia and solid tumors. However, gene transfer to human NK cells is a challenging task, particularly with non-virus-based techniques. Here, we describe a new procedure allowing efficient electroporation-based transfection of plasmid DNA, including CAR and CCR7 genes, in resting or cytokine-expanded human NK cell populations and NK-92 cell line. This procedure may offer a suitable platform for a safe and effective use of CAR-NK cells in adoptive immunotherapy of cancer.
KW - Adoptive immunotherapy
KW - Chemokine receptors
KW - NK cells
KW - Electroporation
KW - Chimeric Antigen Receptors
KW - Adoptive immunotherapy
KW - Chemokine receptors
KW - NK cells
KW - Electroporation
KW - Chimeric Antigen Receptors
UR - http://hdl.handle.net/10807/229474
U2 - 10.3389/fimmu.2019.00957
DO - 10.3389/fimmu.2019.00957
M3 - Article
SN - 1664-3224
VL - 10
SP - 1
EP - 10
JO - Frontiers in Immunology
JF - Frontiers in Immunology
ER -