How to assess drug resistance in cancer stem cells

Maria Laura De Angelis; Ruggero De Maria Marchiano; Marta Baiocchi

doi:10.1007/978-1-4939-7401-6_10

How to assess drug resistance in cancer stem cells

Maria Laura De Angelis, Ruggero De Maria Marchiano, Marta Baiocchi

Istituto Superiore di Sanita

Risultato della ricerca: Contributo in libro › Chapter

4 Citazioni (Scopus)

Abstract

Banks of genetically characterized cancer stem cells (CSCs) isolated from individual patients and grown as spheroids offer an invaluable approach to identify genetic determinants of drug resistance versus sensitivity, and to study new stem cell-directed therapies. Here, we describe our standardized procedure for in vitro drug screening on colorectal CSCs, taking irinotecan as an example.

Lingua originale	English
Titolo della pubblicazione ospite	Cancer Stem Cells: Methods and Protocols, Methods in Molecular Biology,
Pagine	107-115
Numero di pagine	9
Volume	1692
DOI	https://doi.org/10.1007/978-1-4939-7401-6_10
Stato di pubblicazione	Pubblicato - 2018

Serie di pubblicazioni

Nome	METHODS IN MOLECULAR BIOLOGY

Keywords

CSC
Cancer stem cell culture
Colorectal cancer
Drug resistance
Drug screening
Genetics
In vitro assays
Molecular Biology

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abstract = "Banks of genetically characterized cancer stem cells (CSCs) isolated from individual patients and grown as spheroids offer an invaluable approach to identify genetic determinants of drug resistance versus sensitivity, and to study new stem cell-directed therapies. Here, we describe our standardized procedure for in vitro drug screening on colorectal CSCs, taking irinotecan as an example.",

keywords = "CSC, Cancer stem cell culture, Colorectal cancer, Drug resistance, Drug screening, Genetics, In vitro assays, Molecular Biology, CSC, Cancer stem cell culture, Colorectal cancer, Drug resistance, Drug screening, Genetics, In vitro assays, Molecular Biology",

author = "{De Angelis}, {Maria Laura} and {De Maria Marchiano}, Ruggero and Marta Baiocchi",

year = "2018",

doi = "10.1007/978-1-4939-7401-6_10",

language = "English",

isbn = "9781493974009",

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TY - CHAP

T1 - How to assess drug resistance in cancer stem cells

AU - De Angelis, Maria Laura

AU - De Maria Marchiano, Ruggero

AU - Baiocchi, Marta

PY - 2018

Y1 - 2018

N2 - Banks of genetically characterized cancer stem cells (CSCs) isolated from individual patients and grown as spheroids offer an invaluable approach to identify genetic determinants of drug resistance versus sensitivity, and to study new stem cell-directed therapies. Here, we describe our standardized procedure for in vitro drug screening on colorectal CSCs, taking irinotecan as an example.

AB - Banks of genetically characterized cancer stem cells (CSCs) isolated from individual patients and grown as spheroids offer an invaluable approach to identify genetic determinants of drug resistance versus sensitivity, and to study new stem cell-directed therapies. Here, we describe our standardized procedure for in vitro drug screening on colorectal CSCs, taking irinotecan as an example.

KW - CSC

KW - Cancer stem cell culture

KW - Colorectal cancer

KW - Drug resistance

KW - Drug screening

KW - Genetics

KW - In vitro assays

KW - Molecular Biology

KW - CSC

KW - Cancer stem cell culture

KW - Colorectal cancer

KW - Drug resistance

KW - Drug screening

KW - Genetics

KW - In vitro assays

KW - Molecular Biology

UR - http://hdl.handle.net/10807/111830

UR - http://www.springer.com/series/7651

U2 - 10.1007/978-1-4939-7401-6_10

DO - 10.1007/978-1-4939-7401-6_10

M3 - Chapter

SN - 9781493974009

VL - 1692

T3 - METHODS IN MOLECULAR BIOLOGY

SP - 107

EP - 115

BT - Cancer Stem Cells: Methods and Protocols, Methods in Molecular Biology,

ER -

How to assess drug resistance in cancer stem cells

Abstract

Serie di pubblicazioni

Keywords

OSS delle Nazioni Unite

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