TY - JOUR
T1 - HLA-DR is aberrantly expressed at feto-maternal interface in pre-eclampsia
AU - Tersigni, Chiara
AU - Redman, C. W.
AU - Dragovic, R.
AU - Tannetta, D.
AU - Scambia, Giovanni
AU - Di Simone, Nicoletta
AU - Sargent, I.
AU - Vatish, M.
PY - 2018
Y1 - 2018
N2 - In normal pregnancy, villous cytotrophoblast and syncytiotrophoblast do not express HLA Class I and Class II molecules, while invasive extravillous trophoblast only express class I HLA-C and the atypical class Ib antigens, HLA-G, -E and -F. Inadequate maternal tolerance of invasive trophoblast has been proposed as a possible immunologic trigger of poor trophoblast invasion and subsequent occurrence of pre-eclampsia. This study aimed to investigate possible aberrant expression of class II HLA-DR on placentae and syncytiotrophoblast-derived extracellular vesicles (STEVs), obtained by dual placental perfusion, from pre-eclampsia (n = 23) and normal pregnant (n = 14) women. Here we demonstrate that HLA-DR can be detected in syncytiotrophoblast from a significant proportion of pre-eclampsia but not control placentae. HLA-DR was also observed, by flow cytometry, on STEVs and associated with placental alkaline phosphatase to validate their placental origin. HLA-DR positive syncytiotrophoblast was detected in placental biopsies from pre-eclampsia but not normal control cases, using immunohistochemistry. The HLA may be fetal or maternal origin. In the latter case a possible mechanism of acquisition is trogocytosis.
AB - In normal pregnancy, villous cytotrophoblast and syncytiotrophoblast do not express HLA Class I and Class II molecules, while invasive extravillous trophoblast only express class I HLA-C and the atypical class Ib antigens, HLA-G, -E and -F. Inadequate maternal tolerance of invasive trophoblast has been proposed as a possible immunologic trigger of poor trophoblast invasion and subsequent occurrence of pre-eclampsia. This study aimed to investigate possible aberrant expression of class II HLA-DR on placentae and syncytiotrophoblast-derived extracellular vesicles (STEVs), obtained by dual placental perfusion, from pre-eclampsia (n = 23) and normal pregnant (n = 14) women. Here we demonstrate that HLA-DR can be detected in syncytiotrophoblast from a significant proportion of pre-eclampsia but not control placentae. HLA-DR was also observed, by flow cytometry, on STEVs and associated with placental alkaline phosphatase to validate their placental origin. HLA-DR positive syncytiotrophoblast was detected in placental biopsies from pre-eclampsia but not normal control cases, using immunohistochemistry. The HLA may be fetal or maternal origin. In the latter case a possible mechanism of acquisition is trogocytosis.
KW - Extracellular vesicles
KW - Feto-maternal interface
KW - HLA-DR
KW - Pre-eclampsia
KW - Extracellular vesicles
KW - Feto-maternal interface
KW - HLA-DR
KW - Pre-eclampsia
UR - http://hdl.handle.net/10807/128687
U2 - 10.1016/j.jri.2018.06.024
DO - 10.1016/j.jri.2018.06.024
M3 - Article
SN - 0165-0378
SP - 48
EP - 52
JO - Journal of Reproductive Immunology
JF - Journal of Reproductive Immunology
ER -