HIGH-DOSE ATORVASTATIN REDUCE OXIDATIVE STRESS OF ISCHEMIA/REPERFUSION INJURY AFTER ISOGENEIC KIDNEY TRANSPLANTATION IN RATS

Jacopo Romagnoli, Franco Citterio, Giovanna Liuzzo, Filippo Crea, Arnaldo Capelli, Giacomo Cusumano, Giorgia Copponi, Melissa Manchi, Anna Severino, E Cola, Me Caristo

Risultato della ricerca: Contributo in rivistaArticolo in rivista

Abstract

OBJECTIVE: To investigate the effects of N-acetylcysteine (NAC) and high-dose atorvastatin (ATOR) in reducing oxidative stress in a rat kidney model of ischemia-reperfusion injury. METHODS: Forty female rats underwent clamping of the left renal artery for 30 minutes, followed by reperfusion. The effects of pre-ischemic administration of NAC and/or ATOR were evaluated within 4 groups: a) control (no NAC, no ATOR); b) NAC (intraperitoneal NAC administration); c) ATOR (oral ATOR administration); and d) NAC+ATOR (both drugs). Oxidative stress was assessed by measuring the activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and myeloperoxidase (MPO). Post-ischemia-reperfusion injury was evaluated by means of renal histology. RESULTS: NAC, ATOR, and NAC+ATOR in rats showed lower MPO (P < .05) and higher GPx activity (P < .05) versus control; SOD activity was lower in NAC versus ATOR (P < .05). No difference among groups was found at histology. However, a lower rate of tubular ischemic lesions was evident in NAC+ATOR versus control (P = .07). CONCLUSIONS: Atorvastatin pretreatment provides protection against oxidative stress in a rat kidney model of ischemia-reperfusion injury, reinforcing the evidence of a beneficial effect of statins beyond their cholesterol-lowering properties
Lingua originaleEnglish
pagine (da-a)2757-2762
Numero di pagine6
RivistaTransplantation Proceedings
Volume2015
DOI
Stato di pubblicazionePubblicato - 2015

Keywords

  • ISCHEMIA REPERFUSION
  • TRANSPLANTATION

Fingerprint Entra nei temi di ricerca di 'HIGH-DOSE ATORVASTATIN REDUCE OXIDATIVE STRESS OF ISCHEMIA/REPERFUSION INJURY AFTER ISOGENEIC KIDNEY TRANSPLANTATION IN RATS'. Insieme formano una fingerprint unica.

Cita questo