Herpes simplex virus-1 and cytomegalovirus DNAs detection in the inner ear of implanted patients with non-congenital infection

Walter Di Nardo, Rosaria Santangelo, Gaetano Paludetti, Roberta Anzivino, Paola Cattani Franchi, Eugenio De Corso

Risultato della ricerca: Contributo in rivistaArticolo in rivista


Conclusions: The detection of cytomegalovirus (CMV) and herpes simplex virus-1 (HSV-1) genome in perilymph of patients with negative serology or clinical history for congenital infections supports the hypothesis that Herpesviridae, even after acquired postnatal infections, could remain in latent phase in the spiral ganglion and damage the cochlea by a possible subsequent reactivation. Further studies are needed to identify the markers of such reactivation. Objective: To identify the presence of certain viral species in the endolabyrinthic fluid of deaf patients with non-congenital infection. The research of viral DNA within the inner ear is the only direct way to increase our knowledge about the viral role in postnatal damage to the cochlea. Methods: Thirty-six patients (1–69 years) suffering from bilateral sensorineural hearing loss (SNHL) were subjected, during cochlear implant (CI) surgery, to a sample taking of inner ear fluid. Several types of viral genome (HSV, VZV, CMV, EBV and Enterovirus) were investigated in each sample through multiplex polymerase chain reaction (PCR) and reverse transcriptase-polymerase chain reaction (RT-PCR). Radiological exams, serology (specific IgG and IgM) and PCR of peripheral blood were also performed. Results: While the research of the viral genome in peripheral blood was negative in all patients, multiplex PCR on endolabyrinthic fluid samples was positive in three patients (two cases of CMV-DNA and one case of HSV-1 DNA).
Lingua originaleEnglish
pagine (da-a)791-796
Numero di pagine6
RivistaActa Oto-Laryngologica
Stato di pubblicazionePubblicato - 2017


  • Adolescent
  • Adult
  • Aged
  • Child
  • Child, Preschool
  • Cochlear Implantation
  • Cochlear implant
  • Cohort Studies
  • Cytomegalovirus
  • DNA, Viral
  • Endolymph
  • Female
  • Hearing Loss, Sensorineural
  • Herpesvirus 1, Human
  • Humans
  • Infant
  • Male
  • Middle Aged
  • Otorhinolaryngology2734 Pathology and Forensic Medicine
  • PCR
  • Polymerase Chain Reaction
  • RT-PCR
  • Young Adult
  • hearing loss
  • herpesvirus
  • viral genome


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