TY - JOUR
T1 - Hepatocellular-Carcinoma-Derived Organoids: Innovation in Cancer Research
AU - Airola, Carlo
AU - Pallozzi, Maria
AU - Cesari, Eleonora
AU - Cerrito, Lucia
AU - Stella, Leonardo
AU - Sette, Claudio
AU - Giuliante, Felice
AU - Gasbarrini, Antonio
AU - Ponziani, Francesca Romana
PY - 2024
Y1 - 2024
N2 - Hepatocellular carcinomas (HCCs) are highly heterogeneous malignancies. They are characterized by a peculiar tumor microenvironment and dense vascularization. The importance of signaling between immune cells, endothelial cells, and tumor cells leads to the difficult recapitulation of a reliable in vitro HCC model using the conventional two-dimensional cell cultures. The advent of three-dimensional organoid tumor technology has revolutionized our understanding of the pathogenesis and progression of several malignancies by faithfully replicating the original cancer genomic, epigenomic, and microenvironmental landscape. Organoids more closely mimic the in vivo environment and cell interactions, replicating factors such as the spatial organization of cell surface receptors and gene expression, and will probably become an important tool in the choice of therapies and the evaluation of tumor response to treatments. This review aimed to describe the ongoing and potential applications of organoids as an in vitro model for the study of HCC development, its interaction with the host's immunity, the analysis of drug sensitivity tests, and the current limits in this field.
AB - Hepatocellular carcinomas (HCCs) are highly heterogeneous malignancies. They are characterized by a peculiar tumor microenvironment and dense vascularization. The importance of signaling between immune cells, endothelial cells, and tumor cells leads to the difficult recapitulation of a reliable in vitro HCC model using the conventional two-dimensional cell cultures. The advent of three-dimensional organoid tumor technology has revolutionized our understanding of the pathogenesis and progression of several malignancies by faithfully replicating the original cancer genomic, epigenomic, and microenvironmental landscape. Organoids more closely mimic the in vivo environment and cell interactions, replicating factors such as the spatial organization of cell surface receptors and gene expression, and will probably become an important tool in the choice of therapies and the evaluation of tumor response to treatments. This review aimed to describe the ongoing and potential applications of organoids as an in vitro model for the study of HCC development, its interaction with the host's immunity, the analysis of drug sensitivity tests, and the current limits in this field.
KW - drug sensitivity
KW - tumor microenvironment
KW - liver organoids
KW - hepatocellular carcinoma
KW - drug sensitivity
KW - tumor microenvironment
KW - liver organoids
KW - hepatocellular carcinoma
UR - http://hdl.handle.net/10807/299752
U2 - 10.3390/cells13201726
DO - 10.3390/cells13201726
M3 - Article
SN - 2073-4409
VL - 13
SP - 1726
EP - 1744
JO - Cells
JF - Cells
ER -