Heparin-binding hemagglutinin HBHA from Mycobacterium tuberculosis affects actin polymerisation

Giovanni Delogu, C Esposito, D Marasco, E Pedone, R. Berisio

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

22 Citazioni (Scopus)

Abstract

HBHA is a mycobacterial cell surface protein that mediates adhesion to epithelial cells and that has been implicated in the dissemination of Mycobacterium tuberculosis (Mtb) from the site of primary infection. In this work, we demonstrate that HBHA is able to bind G-actin whereas its shorter form, deprived of the lysine-rich C-terminal region (HBHAΔC), does not bind. Consistently, interaction of actin with HBHA is competitive with heparin binding. Notably, we also observe that HBHA, but not HBHAΔC, clearly hampers G-actin polymerisation into F-actin filaments. Since Mtb escapes from the phagosome into the cytosol of host cells, where it can persist and replicate, HBHA is properly localised on the bacterial surface to regulate the dynamic process of cytoskeleton formation driven by actin polymerisation and depolymerisation.
Lingua originaleEnglish
pagine (da-a)339-344
Numero di pagine6
RivistaBiochemical and Biophysical Research Communications
Volume410
DOI
Stato di pubblicazionePubblicato - 2011

Keywords

  • Actins
  • Humans
  • Lectins
  • Mycobacterium tuberculosis
  • Phagosomes
  • Protein Conformation

Fingerprint Entra nei temi di ricerca di 'Heparin-binding hemagglutinin HBHA from Mycobacterium tuberculosis affects actin polymerisation'. Insieme formano una fingerprint unica.

Cita questo