TY - JOUR
T1 - Head bobber: an insertional mutation causes inner ear defects, hyperactive circling, and deafness
AU - Somma, Giuseppina
AU - Alger, Heather M.
AU - Mcguire, Ryan M.
AU - Kretlow, Jim D.
AU - R.Ruiz, Fernanda
AU - Yatsenko, Svetlana A.
AU - Stankiewicz, Pawel
AU - Harrison, Wilbur
AU - Funk, Etai
AU - Bergamaschi, Antonio
AU - Oghalai, John S.
AU - Mikos, Antonios G.
AU - Overbeek, Paul A.
AU - Pereira, Fred A.
PY - 2012
Y1 - 2012
N2 - The head bobber transgenic mouse line, produced by pronuclear integration, exhibits repetitive head tilting, circling behavior, and severe hearing loss. Transmitted as an autosomal recessive trait, the homozygote has vestibular and cochlea inner ear defects. The space between the semicircular canals is enclosed within the otic capsule creating a vacuous chamber with remnants of the semicircular canals, associated cristae, and vestibular organs. A poorly developed stria vascularis and endolymphatic duct is likely the cause for Reissner's membrane to collapse post-natally onto the organ of Corti in the cochlea. Molecular analyses identified a single integration of ~3 tandemly repeated copies of the transgene, a short duplicated segment of chromosome X and a 648 kb deletion of chromosome 7(F3). The three known genes (Gpr26, Cpxm2, and Chst15) in the deleted region are conserved in mammals and expressed in the wild-type inner ear during vestibular and cochlea development but are absent in homozygous mutant ears. We propose that genes critical for inner ear patterning and differentiation are lost at the head bobber locus and are candidate genes for human deafness and vestibular disorders.
AB - The head bobber transgenic mouse line, produced by pronuclear integration, exhibits repetitive head tilting, circling behavior, and severe hearing loss. Transmitted as an autosomal recessive trait, the homozygote has vestibular and cochlea inner ear defects. The space between the semicircular canals is enclosed within the otic capsule creating a vacuous chamber with remnants of the semicircular canals, associated cristae, and vestibular organs. A poorly developed stria vascularis and endolymphatic duct is likely the cause for Reissner's membrane to collapse post-natally onto the organ of Corti in the cochlea. Molecular analyses identified a single integration of ~3 tandemly repeated copies of the transgene, a short duplicated segment of chromosome X and a 648 kb deletion of chromosome 7(F3). The three known genes (Gpr26, Cpxm2, and Chst15) in the deleted region are conserved in mammals and expressed in the wild-type inner ear during vestibular and cochlea development but are absent in homozygous mutant ears. We propose that genes critical for inner ear patterning and differentiation are lost at the head bobber locus and are candidate genes for human deafness and vestibular disorders.
KW - ear defects
KW - ear defects
UR - http://hdl.handle.net/10807/3709
U2 - 10.1007/s10162-012-0316-5
DO - 10.1007/s10162-012-0316-5
M3 - Article
VL - 13
SP - 335
EP - 349
JO - Journal of the Association for Research in Otolaryngology : JARO
JF - Journal of the Association for Research in Otolaryngology : JARO
ER -