Hb(αα,ββ): a novel fusion construct for a dimeric, four domain hemoglobin

Gianna Panetta, Alessandro Arcovito, Veronica Morea, Andrea Bellelli, Adriana Erica Miele

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13 Citazioni (Scopus)


Hemoglobin-based blood substitutes are one of the options available to derive a resuscitating fluid taking into account clinical and physiological demands. In this paper we investigated a novel protein, Hb(αα,ββ) obtained as a combination of two homodimers α2 and β2 both derived from a fusion gene containing two alfa chains or two beta chains respectively coupled via a specific linker. The construct here described is thus a novel heterodimeric hemoglobin carrying four heme groups. The protein cannot dissociate into dimers, as demonstrated by its absence of reactivity versus haptoglobin, and is expected to have a relatively long circulating half-life. The modification does not increase the autoxidation rate, but increases the oxygen affinity, due to a destabilization of the T quaternary state. Characterization of the biochemical properties of this protein in comparison with HbA is reported.
Lingua originaleEnglish
pagine (da-a)1462-1470
Numero di pagine9
Stato di pubblicazionePubblicato - 2008


  • blood substitutes
  • gene fusion
  • oxygen carrier
  • protein engineering


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