Hb(αα,ββ): a novel fusion construct for a dimeric, four domain hemoglobin

Alessandro Arcovito, Gianna Panetta, Veronica Morea, Andrea Bellelli, Adriana Erica Miele

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

13 Citazioni (Scopus)

Abstract

Hemoglobin-based blood substitutes are one of the options available to derive a resuscitating fluid taking into account clinical and physiological demands. In this paper we investigated a novel protein, Hb(αα,ββ) obtained as a combination of two homodimers α2 and β2 both derived from a fusion gene containing two alfa chains or two beta chains respectively coupled via a specific linker. The construct here described is thus a novel heterodimeric hemoglobin carrying four heme groups. The protein cannot dissociate into dimers, as demonstrated by its absence of reactivity versus haptoglobin, and is expected to have a relatively long circulating half-life. The modification does not increase the autoxidation rate, but increases the oxygen affinity, due to a destabilization of the T quaternary state. Characterization of the biochemical properties of this protein in comparison with HbA is reported.
Lingua originaleEnglish
pagine (da-a)1462-1470
Numero di pagine9
RivistaBIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
Stato di pubblicazionePubblicato - 2008

Keywords

  • blood substitutes
  • gene fusion
  • oxygen carrier
  • protein engineering

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