Glutathione peroxidase responses in mature horses following the withdrawal of an organic selenium supplement.

The study comprised 25 mature horses and incorporated five dietary treatments; a negative control\r\n(C: 0.085 ppm of Se in the diet), 3 levels of organic Se supplementation (S2, S3 and S4; 0.2, 0.3 and 0.4 ppm of\r\nSe on DM respectively) and one positive control supplemented with Na selenite (N3: 0.3 ppm of Se on DM). Diets\r\nwere offered for 112 days (supplementary period) after which horses were blood sampled to determine GSH-Px\r\nactivity. Sampling was repeated at 28 day intervals for the following 112 days (post-supplementary period).\r\nIncreased GSH-Px activity was observed with ascending concentrations of dietary Se following the supplementary\r\nperiod. GSH-Px activities are still notably higher at the end of the post-supplementary period in S2, S3, S4 and N3\r\nwhen compared to C, although rates of decline were greatest in those treatments with the highest levels of selenium\r\nsupplementation. Furthermore, GSH-Px activity was better maintained in those animals that had received\r\norganic Se following the withdrawal of Se supplements. Therefore, from a nutritional viewpoint, organic forms of\r\nSe are superior to selenite as GSH-Px activity is maintained during periods of Se inadequacy.