TY - JOUR
T1 - Glucocorticoid treatment reduces T-bet and pSTAT1 expression in mononuclear cells from relapsing remitting multiple sclerosis patients.
AU - Frisullo, Giovanni
AU - Nociti, Viviana
AU - Iorio, Raffaele
AU - Patanella, Agata Katia
AU - Bianco, Assunta
AU - Sancricca, Cristina
AU - Caggiula, Marcella
AU - Tonali, Pietro Attilio
AU - Mirabella, Massimiliano
AU - Batocchi, Anna Paola
PY - 2007
Y1 - 2007
N2 - High dose glucocorticoid (GC) treatment has been demonstrated to have a short-term beneficial effect on functional recovery in relapsing multiple sclerosis (MS) patients but the exact mechanism of action of GCs in MS is unclear. We found that high dose intravenous GCs strongly reduced T-bet and pSTAT1 expression in CD4+, CD8+, CD14+ circulating cells in RRMS patients in relapse. pSTAT1and T-bet reduction was associated with the decline of IFNgamma production by PBMCs. A significant increase of AV-positive CD4+ and CD8+ T cells was detectable after GC treatment without any variation in the percentage of annexin V-positive monocytes. By in vitro analysis, patients during relapse, either before or after GC treatment, exhibited a lower proportion of apoptotic lymphocytes than remitting patients and controls. Our study suggests that GCs can modulate T-bet and STAT1 expression and that IFNgamma signalling inhibition contributes to anti-inflammatory action of GCs in the treatment of relapses of MS patients
AB - High dose glucocorticoid (GC) treatment has been demonstrated to have a short-term beneficial effect on functional recovery in relapsing multiple sclerosis (MS) patients but the exact mechanism of action of GCs in MS is unclear. We found that high dose intravenous GCs strongly reduced T-bet and pSTAT1 expression in CD4+, CD8+, CD14+ circulating cells in RRMS patients in relapse. pSTAT1and T-bet reduction was associated with the decline of IFNgamma production by PBMCs. A significant increase of AV-positive CD4+ and CD8+ T cells was detectable after GC treatment without any variation in the percentage of annexin V-positive monocytes. By in vitro analysis, patients during relapse, either before or after GC treatment, exhibited a lower proportion of apoptotic lymphocytes than remitting patients and controls. Our study suggests that GCs can modulate T-bet and STAT1 expression and that IFNgamma signalling inhibition contributes to anti-inflammatory action of GCs in the treatment of relapses of MS patients
KW - corticosteroids
KW - multiple sclerosis
KW - transcription factor
KW - corticosteroids
KW - multiple sclerosis
KW - transcription factor
UR - http://hdl.handle.net/10807/7728
U2 - 10.1016/j.clim.2007.05.011
DO - 10.1016/j.clim.2007.05.011
M3 - Article
SN - 1521-6616
VL - 124
SP - 284
EP - 293
JO - Clinical Immunology
JF - Clinical Immunology
ER -