Germ cell apoptosis control during spermatogenesis

C Giampietri, S Petrungaro, P Coluccia, Alessio D'Alessio, D Starace, A Riccioli, F Padula, F Palombi, E Ziparo, A Filippini, P. De Cesaris

Risultato della ricerca: Contributo in rivistaArticolo in rivista

31 Citazioni (Scopus)


The aim of the present study was to investigate the expression and role of c-Flip long isoform (c-FlipL), a known anti-apoptotic protein. No data are currently available on c-FlipL in male gonad before puberty; therefore, this study was carried out in prepuberal mouse testis. We investigated pachytene spermatocytes and spermatogonia by immunostaining of testis sections and found a strong and specific expression of c-FlipL in pachytene spermatocytes, while spermatogonia expressed very low levels of c-FlipL. This finding inversely correlated with the caspases activity, which was higher in spermatogonia as compared to pachytene spermatocytes. Other experiments carried out in an organ-culture model revealed that Fas-induced apoptosis was higher in spermatogonia as compared to pachytene spermatocytes. These data suggest that c-FlipL may play a role as an anti-apoptotic molecule in the prepuberal mouse testis and open new perspectives in the comprehension of the mechanisms controlling germ cells apoptosis.
Lingua originaleEnglish
pagine (da-a)298-302
Numero di pagine5
Stato di pubblicazionePubblicato - 2005


  • Apoptosis
  • CASP8 and FADD-Like Apoptosis Regulating Protein
  • Caspases
  • Cell Differentiation
  • Immunohistochemistry
  • Mice
  • Organ Culture Techniques
  • Sexual Maturation
  • Spermatocytes
  • Spermatogenesis
  • Spermatogonia
  • Spermatozoa
  • Testis


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