Germ cell apoptosis control during spermatogenesis

C Giampietri; S Petrungaro; P Coluccia; Alessio D'Alessio; D Starace; A Riccioli; F Padula; F Palombi; E Ziparo; A Filippini; P. De Cesaris

doi:10.1016/j.contraception.2005.04.011

Germ cell apoptosis control during spermatogenesis

C Giampietri, S Petrungaro, P Coluccia, Alessio D'Alessio, D Starace, A Riccioli, F Padula, F Palombi, E Ziparo, A Filippini, P. De Cesaris

Risultato della ricerca: Contributo in rivista › Articolo in rivista

31 Citazioni (Scopus)

Abstract

The aim of the present study was to investigate the expression and role of c-Flip long isoform (c-FlipL), a known anti-apoptotic protein. No data are currently available on c-FlipL in male gonad before puberty; therefore, this study was carried out in prepuberal mouse testis. We investigated pachytene spermatocytes and spermatogonia by immunostaining of testis sections and found a strong and specific expression of c-FlipL in pachytene spermatocytes, while spermatogonia expressed very low levels of c-FlipL. This finding inversely correlated with the caspases activity, which was higher in spermatogonia as compared to pachytene spermatocytes. Other experiments carried out in an organ-culture model revealed that Fas-induced apoptosis was higher in spermatogonia as compared to pachytene spermatocytes. These data suggest that c-FlipL may play a role as an anti-apoptotic molecule in the prepuberal mouse testis and open new perspectives in the comprehension of the mechanisms controlling germ cells apoptosis.

Lingua originale	English
pagine (da-a)	298-302
Numero di pagine	5
Rivista	Contraception
Volume	72
DOI	https://doi.org/10.1016/j.contraception.2005.04.011
Stato di pubblicazione	Pubblicato - 2005

Keywords

Apoptosis
CASP8 and FADD-Like Apoptosis Regulating Protein
Caspases
Cell Differentiation
Immunohistochemistry
Mice
Organ Culture Techniques
Sexual Maturation
Spermatocytes
Spermatogenesis
Spermatogonia
Spermatozoa
Testis

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title = "Germ cell apoptosis control during spermatogenesis",

abstract = "The aim of the present study was to investigate the expression and role of c-Flip long isoform (c-FlipL), a known anti-apoptotic protein. No data are currently available on c-FlipL in male gonad before puberty; therefore, this study was carried out in prepuberal mouse testis. We investigated pachytene spermatocytes and spermatogonia by immunostaining of testis sections and found a strong and specific expression of c-FlipL in pachytene spermatocytes, while spermatogonia expressed very low levels of c-FlipL. This finding inversely correlated with the caspases activity, which was higher in spermatogonia as compared to pachytene spermatocytes. Other experiments carried out in an organ-culture model revealed that Fas-induced apoptosis was higher in spermatogonia as compared to pachytene spermatocytes. These data suggest that c-FlipL may play a role as an anti-apoptotic molecule in the prepuberal mouse testis and open new perspectives in the comprehension of the mechanisms controlling germ cells apoptosis.",

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author = "C Giampietri and S Petrungaro and P Coluccia and Alessio D'Alessio and D Starace and A Riccioli and F Padula and F Palombi and E Ziparo and A Filippini and {De Cesaris}, P.",

year = "2005",

doi = "10.1016/j.contraception.2005.04.011",

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volume = "72",

pages = "298--302",

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TY - JOUR

T1 - Germ cell apoptosis control during spermatogenesis

AU - Giampietri, C

AU - Petrungaro, S

AU - Coluccia, P

AU - D'Alessio, Alessio

AU - Starace, D

AU - Riccioli, A

AU - Padula, F

AU - Palombi, F

AU - Ziparo, E

AU - Filippini, A

AU - De Cesaris, P.

PY - 2005

Y1 - 2005

N2 - The aim of the present study was to investigate the expression and role of c-Flip long isoform (c-FlipL), a known anti-apoptotic protein. No data are currently available on c-FlipL in male gonad before puberty; therefore, this study was carried out in prepuberal mouse testis. We investigated pachytene spermatocytes and spermatogonia by immunostaining of testis sections and found a strong and specific expression of c-FlipL in pachytene spermatocytes, while spermatogonia expressed very low levels of c-FlipL. This finding inversely correlated with the caspases activity, which was higher in spermatogonia as compared to pachytene spermatocytes. Other experiments carried out in an organ-culture model revealed that Fas-induced apoptosis was higher in spermatogonia as compared to pachytene spermatocytes. These data suggest that c-FlipL may play a role as an anti-apoptotic molecule in the prepuberal mouse testis and open new perspectives in the comprehension of the mechanisms controlling germ cells apoptosis.

AB - The aim of the present study was to investigate the expression and role of c-Flip long isoform (c-FlipL), a known anti-apoptotic protein. No data are currently available on c-FlipL in male gonad before puberty; therefore, this study was carried out in prepuberal mouse testis. We investigated pachytene spermatocytes and spermatogonia by immunostaining of testis sections and found a strong and specific expression of c-FlipL in pachytene spermatocytes, while spermatogonia expressed very low levels of c-FlipL. This finding inversely correlated with the caspases activity, which was higher in spermatogonia as compared to pachytene spermatocytes. Other experiments carried out in an organ-culture model revealed that Fas-induced apoptosis was higher in spermatogonia as compared to pachytene spermatocytes. These data suggest that c-FlipL may play a role as an anti-apoptotic molecule in the prepuberal mouse testis and open new perspectives in the comprehension of the mechanisms controlling germ cells apoptosis.

KW - Apoptosis

KW - CASP8 and FADD-Like Apoptosis Regulating Protein

KW - Caspases

KW - Cell Differentiation

KW - Immunohistochemistry

KW - Mice

KW - Organ Culture Techniques

KW - Sexual Maturation

KW - Spermatocytes

KW - Spermatogenesis

KW - Spermatogonia

KW - Spermatozoa

KW - Testis

KW - Apoptosis

KW - CASP8 and FADD-Like Apoptosis Regulating Protein

KW - Caspases

KW - Cell Differentiation

KW - Immunohistochemistry

KW - Mice

KW - Organ Culture Techniques

KW - Sexual Maturation

KW - Spermatocytes

KW - Spermatogenesis

KW - Spermatogonia

KW - Spermatozoa

KW - Testis

UR - http://hdl.handle.net/10807/13530

U2 - 10.1016/j.contraception.2005.04.011

DO - 10.1016/j.contraception.2005.04.011

M3 - Article

SN - 0010-7824

VL - 72

SP - 298

EP - 302

JO - Contraception

JF - Contraception

ER -

Germ cell apoptosis control during spermatogenesis

Abstract

Keywords

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