Genotypic testing on HIV-1 DNA as a tool to assess HIV-1 co-receptor usage in clinical practice: results from the DIVA study group

V Svicher; C Alteri; M Montano; A Nori; R D'Arrigo; M Andreoni; G Angarano; A Antinori; G Antonelli; T Allice; P Bagnarelli; F Baldanti; A Bertoli; M Borderi; E Boeri; I Bon; B Bruzzone; R Barresi; S Calderisi; Ap Callegaro; Capobianchi; F Gargiulo; F Castelli; Roberto Cauda; F Ceccherini Silberstein; M Clementi; A Chirianni; Manuela Colafigli; A D'Arminio Monforte; Andrea De Luca; A Di Biagio; G Di Nicuolo; G Di Perri; F Di Santo; G Fadda; M Galli; W Gennari; V Ghisetti; A Costantini; A Gori; R Gulminetti; F Leoncini; G Maffongelli; F Maggiolo; R Maserati; F Mazzotta; G Meini; V Micheli; L Monno; C Mussini; S Nozza; S Paolucci; G Palù; S Parisi; G Parruti; Ar Pignataro; T Quirino; Mc Re; G Rizzardini; Maurizio Sanguinetti; Rosaria Santangelo; R Scaggiante; G Sterrantino; O Turriziani; Ml Vatteroni; C Viscoli; V Vullo; M Zazzi; A Lazzarin; Cf Perno

doi:10.1007/s15010-013-0510-3

Genotypic testing on HIV-1 DNA as a tool to assess HIV-1 co-receptor usage in clinical practice: results from the DIVA study group

V Svicher
, C Alteri
, M Montano
, A Nori
, R D'Arrigo
, M Andreoni
, G Angarano
, A Antinori
, G Antonelli
, T Allice
, P Bagnarelli
, F Baldanti
, A Bertoli
, M Borderi
, E Boeri
, I Bon
, B Bruzzone
, R Barresi
, S Calderisi
, Ap Callegaro

Capobianchi, F Gargiulo, F Castelli, Roberto Cauda, F Ceccherini Silberstein, M Clementi, A Chirianni, Manuela Colafigli, A D'Arminio Monforte, Andrea De Luca, A Di Biagio, G Di Nicuolo, G Di Perri, F Di Santo, G Fadda, M Galli, W Gennari, V Ghisetti, A Costantini, A Gori, R Gulminetti, F Leoncini, G Maffongelli, F Maggiolo, R Maserati, F Mazzotta, G Meini, V Micheli, L Monno, C Mussini, S Nozza, S Paolucci, G Palù, S Parisi, G Parruti, Ar Pignataro, T Quirino, Mc Re, G Rizzardini, Maurizio Sanguinetti, Rosaria Santangelo, R Scaggiante, G Sterrantino, O Turriziani, Ml Vatteroni, C Viscoli, V Vullo, M Zazzi, A Lazzarin, Cf Perno^*

^*Autore corrispondente per questo lavoro

Risultato della ricerca: Contributo in rivista › Articolo › peer review

9 Citazioni (Scopus)

Abstract

We have developed a sequencing assay for determining the usage of the genotypic HIV-1 co-receptor using peripheral blood mononuclear cell (PBMC) DNA in virologically suppressed HIV-1 infected patients. Our specific aims were to (1) evaluate the efficiency of V3 sequences in B versus non-B subtypes, (2) compare the efficiency of V3 sequences and tropism prediction using whole blood and PBMCs for DNA extraction, (3) compare the efficiency of V3 sequences and tropism prediction using a single versus a triplicate round of amplification.

Lingua originale	Inglese
pagine (da-a)	61-71
Numero di pagine	11
Rivista	Infection
Volume	42
Numero di pubblicazione	1
DOI	https://doi.org/10.1007/s15010-013-0510-3
Stato di pubblicazione	Pubblicato - 2014

All Science Journal Classification (ASJC) codes

Microbiologia (medica)
Malattie Infettive

Keywords

HIV1-DNA
genotypic
tropism

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10.1007/s15010-013-0510-3

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Svicher, V., Alteri, C., Montano, M., Nori, A., D'Arrigo, R., Andreoni, M., Angarano, G., Antinori, A., Antonelli, G., Allice, T., Bagnarelli, P., Baldanti, F., Bertoli, A., Borderi, M., Boeri, E., Bon, I., Bruzzone, B., Barresi, R., Calderisi, S., ... Perno, C. (2014). Genotypic testing on HIV-1 DNA as a tool to assess HIV-1 co-receptor usage in clinical practice: results from the DIVA study group. Infection, 42(1), 61-71. https://doi.org/10.1007/s15010-013-0510-3

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abstract = "We have developed a sequencing assay for determining the usage of the genotypic HIV-1 co-receptor using peripheral blood mononuclear cell (PBMC) DNA in virologically suppressed HIV-1 infected patients. Our specific aims were to (1) evaluate the efficiency of V3 sequences in B versus non-B subtypes, (2) compare the efficiency of V3 sequences and tropism prediction using whole blood and PBMCs for DNA extraction, (3) compare the efficiency of V3 sequences and tropism prediction using a single versus a triplicate round of amplification.",

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author = "V Svicher and C Alteri and M Montano and A Nori and R D'Arrigo and M Andreoni and G Angarano and A Antinori and G Antonelli and T Allice and P Bagnarelli and F Baldanti and A Bertoli and M Borderi and E Boeri and I Bon and B Bruzzone and R Barresi and S Calderisi and Ap Callegaro and Capobianchi and F Gargiulo and F Castelli and Roberto Cauda and \{Ceccherini Silberstein\}, F and M Clementi and A Chirianni and Manuela Colafigli and \{D'Arminio Monforte\}, A and \{De Luca\}, Andrea and \{Di Biagio\}, A and \{Di Nicuolo\}, G and \{Di Perri\}, G and \{Di Santo\}, F and G Fadda and M Galli and W Gennari and V Ghisetti and A Costantini and A Gori and R Gulminetti and F Leoncini and G Maffongelli and F Maggiolo and R Maserati and F Mazzotta and G Meini and V Micheli and L Monno and C Mussini and S Nozza and S Paolucci and G Pal{\`u} and S Parisi and G Parruti and Ar Pignataro and T Quirino and Mc Re and G Rizzardini and Maurizio Sanguinetti and Rosaria Santangelo and R Scaggiante and G Sterrantino and O Turriziani and Ml Vatteroni and C Viscoli and V Vullo and M Zazzi and A Lazzarin and Cf Perno",

year = "2014",

doi = "10.1007/s15010-013-0510-3",

language = "English",

volume = "42",

pages = "61--71",

journal = "Infection",

issn = "0300-8126",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "1",

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Svicher, V, Alteri, C, Montano, M, Nori, A, D'Arrigo, R, Andreoni, M, Angarano, G, Antinori, A, Antonelli, G, Allice, T, Bagnarelli, P, Baldanti, F, Bertoli, A, Borderi, M, Boeri, E, Bon, I, Bruzzone, B, Barresi, R, Calderisi, S, Callegaro, A, Capobianchi, Gargiulo, F, Castelli, F, Cauda, R, Ceccherini Silberstein, F, Clementi, M, Chirianni, A, Colafigli, M, D'Arminio Monforte, A, De Luca, A, Di Biagio, A, Di Nicuolo, G, Di Perri, G, Di Santo, F, Fadda, G, Galli, M, Gennari, W, Ghisetti, V, Costantini, A, Gori, A, Gulminetti, R, Leoncini, F, Maffongelli, G, Maggiolo, F, Maserati, R, Mazzotta, F, Meini, G, Micheli, V, Monno, L, Mussini, C, Nozza, S, Paolucci, S, Palù, G, Parisi, S, Parruti, G, Pignataro, A, Quirino, T, Re, M, Rizzardini, G, Sanguinetti, M , Santangelo, R, Scaggiante, R, Sterrantino, G, Turriziani, O, Vatteroni, M, Viscoli, C, Vullo, V, Zazzi, M, Lazzarin, A & Perno, C 2014, 'Genotypic testing on HIV-1 DNA as a tool to assess HIV-1 co-receptor usage in clinical practice: results from the DIVA study group', Infection, vol. 42, n. 1, pagg. 61-71. https://doi.org/10.1007/s15010-013-0510-3

TY - JOUR

T1 - Genotypic testing on HIV-1 DNA as a tool to assess HIV-1 co-receptor usage in clinical practice: results from the DIVA study group

AU - Svicher, V

AU - Alteri, C

AU - Montano, M

AU - Nori, A

AU - D'Arrigo, R

AU - Andreoni, M

AU - Angarano, G

AU - Antinori, A

AU - Antonelli, G

AU - Allice, T

AU - Bagnarelli, P

AU - Baldanti, F

AU - Bertoli, A

AU - Borderi, M

AU - Boeri, E

AU - Bon, I

AU - Bruzzone, B

AU - Barresi, R

AU - Calderisi, S

AU - Callegaro, Ap

AU - Capobianchi,

AU - Gargiulo, F

AU - Castelli, F

AU - Cauda, Roberto

AU - Ceccherini Silberstein, F

AU - Clementi, M

AU - Chirianni, A

AU - Colafigli, Manuela

AU - D'Arminio Monforte, A

AU - De Luca, Andrea

AU - Di Biagio, A

AU - Di Nicuolo, G

AU - Di Perri, G

AU - Di Santo, F

AU - Fadda, G

AU - Galli, M

AU - Gennari, W

AU - Ghisetti, V

AU - Costantini, A

AU - Gori, A

AU - Gulminetti, R

AU - Leoncini, F

AU - Maffongelli, G

AU - Maggiolo, F

AU - Maserati, R

AU - Mazzotta, F

AU - Meini, G

AU - Micheli, V

AU - Monno, L

AU - Mussini, C

AU - Nozza, S

AU - Paolucci, S

AU - Palù, G

AU - Parisi, S

AU - Parruti, G

AU - Pignataro, Ar

AU - Quirino, T

AU - Re, Mc

AU - Rizzardini, G

AU - Sanguinetti, Maurizio

AU - Santangelo, Rosaria

AU - Scaggiante, R

AU - Sterrantino, G

AU - Turriziani, O

AU - Vatteroni, Ml

AU - Viscoli, C

AU - Vullo, V

AU - Zazzi, M

AU - Lazzarin, A

AU - Perno, Cf

PY - 2014

Y1 - 2014

N2 - We have developed a sequencing assay for determining the usage of the genotypic HIV-1 co-receptor using peripheral blood mononuclear cell (PBMC) DNA in virologically suppressed HIV-1 infected patients. Our specific aims were to (1) evaluate the efficiency of V3 sequences in B versus non-B subtypes, (2) compare the efficiency of V3 sequences and tropism prediction using whole blood and PBMCs for DNA extraction, (3) compare the efficiency of V3 sequences and tropism prediction using a single versus a triplicate round of amplification.

AB - We have developed a sequencing assay for determining the usage of the genotypic HIV-1 co-receptor using peripheral blood mononuclear cell (PBMC) DNA in virologically suppressed HIV-1 infected patients. Our specific aims were to (1) evaluate the efficiency of V3 sequences in B versus non-B subtypes, (2) compare the efficiency of V3 sequences and tropism prediction using whole blood and PBMCs for DNA extraction, (3) compare the efficiency of V3 sequences and tropism prediction using a single versus a triplicate round of amplification.

KW - HIV1-DNA

KW - genotypic

KW - tropism

KW - HIV1-DNA

KW - genotypic

KW - tropism

UR - https://publicatt.unicatt.it/handle/10807/52366

UR - https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84898546832&origin=inward

UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84898546832&origin=inward

U2 - 10.1007/s15010-013-0510-3

DO - 10.1007/s15010-013-0510-3

M3 - Article

SN - 0300-8126

VL - 42

SP - 61

EP - 71

JO - Infection

JF - Infection

IS - 1

ER -

Genotypic testing on HIV-1 DNA as a tool to assess HIV-1 co-receptor usage in clinical practice: results from the DIVA study group

Abstract

All Science Journal Classification (ASJC) codes

Keywords

OSS delle Nazioni Unite

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