Genome wide DNA-profiling of HIV-related B-cell lymphomas

Maurizio Martini, Luigi Maria Larocca, Daniela Capello, Marta Scandurra, Giulia Poretti, Paola Rancoita, Michael Mian, Annunziata Gloghini, Clara Deambrogi, Davide Rossi, Timothy Greine, Chan Wing, Maurilio Ponzoni, Santiago Moreno, Miguel Piris, Vincenzo Canzonieri, Michele Spina, Umberto Tirelli, Giorgio Inghirami, Andrea RinaldiEmanuela Zucca, Riccardo Dalla Favera, Franco Cavalli, Ivo Kwee, Antonino Carbone, Gianluca Gaidano, Francesco Bertoni

Risultato della ricerca: Contributo in rivistaArticolo in rivista

51 Citazioni (Scopus)

Abstract

Non-Hodgkin lymphomas (NHL) represent a frequent complication of human immunodeficiency virus (HIV) infection. To elucidate HIV-NHL pathogenesis, we performed a genome-wide DNA profiling based on a single nucleotide polymorphism-based microarray comparative genomic hybridization in 57 HIV-lymphomas and, for comparison, in 105 immunocompetent diffuse large B-cell lymphomas (IC-DLBCL). Genomic complexity varied across HIV-NHL subtypes. HIV-Burkitt lymphoma showed a significantly lower number of lesions than HIV-DLBCL (P = 0.032), whereas the median number of copy number changes was significantly higher in Epstein-Barr virus negative (EBV-) HIV-DLBCL (42.5, range 8-153) compared to EBV+ cases (22; range 3-41; P = 0.029). Compared to IC-DLBCL, HIV-DLBCL displayed a distinct genomic profile with no gains of 18q and specific genetic lesions. Fragile sites-associated genes, including FHIT (FRA3B), WWOX (FRA16D), DCC (FRA18B) and PARK2 (FRA6E) were frequently inactivated in HIV-NHL by interstitial deletions, and a significantly higher prevalence of FHIT alterations was observed in HIV-DLBCL compared to IC-DLBCL. The same genes involved by fragile site deletions were also frequently affected by aberrant methylation of regulative regions.
Lingua originaleEnglish
pagine (da-a)245-255
Numero di pagine11
RivistaBritish Journal of Haematology
Volume148
DOI
Stato di pubblicazionePubblicato - 2010

Keywords

  • Burkitt Lymphoma
  • Chromosome Aberrations
  • DNA Methylation
  • Gene Frequency
  • Humans
  • Lymphoma, AIDS-Related
  • Lymphoma, Large B-Cell, Diffuse
  • Microarray Analysis
  • Polymerase Chain Reaction
  • Polymorphism, Single Nucleotide

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