Genome-based studies indicate that the enterococcus faecium clade b strains belong to enterococcus lactis species and lack of the hospital infection associated markers

Daniela Bassi, Pier Sandro Cocconcelli, Mireya Viviana Belloso Daza, Claudia Cortimiglia

Risultato della ricerca: Contributo in rivistaArticolo in rivista

Abstract

Enterococcus lactis and the heterotypic synonym Enterococcus xinjiangensis from dairy origin have recently been identified as a novel species based on 16S rRNA gene sequence analysis. Enterococcus faecium type strain NCTC 7171T was used as the reference genome for determining E. lactis and E. faecium to be separate species. However, this taxonomic classification did not consider the diverse lineages of E. faecium, and the double nature of hospital-associated (clade A) and community-associated (clade B) isolates. Here, we investigated the taxonomic relationship among isolates of E. faecium of different origins and E. lactis, using a genome-based approach. Additional to 16S rRNA gene sequence analysis, we estimated the relatedness among strains and species using phylogenomics based on the core pangenome, multilocus sequence typing, the average nucleotide identity and digital DNA–DNA hybridization. Moreover, following the available safety assessment schemes, we evaluated the virulence profile and the ampicillin resistance of E. lactis and E. faecium clade B strains. Our results confirmed the genetic and evolution-ary differences between clade A and the intertwined clade B and E. lactis group. We also confirmed the absence in these strains of virulence gene markers IS16, hylEfm and esp and the lack of the PBP5 allelic profile associated with ampicillin resistance. Taken together, our findings support the reassignment of the strains of E. faecium clade B as E. lactis.
Lingua originaleEnglish
pagine (da-a)1-10
Numero di pagine10
RivistaInternational Journal of Systematic and Evolutionary Microbiology
Volume71
DOI
Stato di pubblicazionePubblicato - 2021

Keywords

  • Anti-Bacterial Agents
  • Bacterial Typing Techniques
  • Base Composition
  • Clade B
  • Cross Infection
  • DNA, Bacterial
  • Enterococcus
  • Enterococcus faecium
  • Enterococcus lactis
  • Fatty Acids
  • Humans
  • Phylogeny
  • RNA, Ribosomal, 16S
  • Sequence Analysis, DNA
  • Virulence markers
  • Whole-genome based studies

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