Gene expression signature for angiogenic and non angiogenic non small cell lung cancer.

Stefano Margaritora, Alessandro Bianchi, Alfredo Cesario, Pierluigi Granone, Jiangting Hu, Mark Ferguson, Peter Goldstraw, Mark Tetlow, Cathy Ratcliffe, Andrew Nicholson, Adrian Harris, Kevin Gatter, Francesco Pezzella

Risultato della ricerca: Contributo in rivistaArticolo in rivista

Abstract

Angiogenesis is regarded as essential for tumour growth. However, we have demonstrated that some other aggressive non-small-cell lung carcinomas (n-SCLC) do not have angiogenesis. In this study, using cDNA microarray analysis, we demonstrate that angiogenic and nonangiogenic tumour types can be distinguished by their gene expression profiles. Tissue samples from 42 n-SCLC patients were obtained with consent. In all, 12 tumours were nonangiogenic and 30 angiogenic. The two groups were matched by age, sex, smoking and tumour stage. Total RNAs were extracted followed by microarray hybridization and image scan procedure. Data were analysed using GeneSpring 5.1 software. A total of 62 genes were found to be able to separate angiogenic from nonangiogenic tumours. Nonangiogenic tumours have higher levels of genes concerned with mitochondrial metabolism, mRNA transcription, protein synthesis and the cell cycle. Angiogenic tumours have higher levels of genes coding for membrane vesicles, integrins, remodelling, angiogenesis and apoptosis. These results further support our first finding that nonangiogenic lung tumours are fast-growing tumours filling the alveoli in the absence of vascular remodelling. We raise the hypothesis that in nonangiogenic tumours, hypoxia leads to a higher activation of the mitochondrial respiratory chain, which allows tumour growth without triggering angiogenesis.
Lingua originaleEnglish
pagine (da-a)1212-1219
Numero di pagine8
RivistaOncogene
Stato di pubblicazionePubblicato - 2004

Keywords

  • angiogenesis
  • gene expression
  • lung cancer
  • micro-array

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