Functionally rodless mice: transgenic models for the investigation of cone function in retinal disease and therapy. Vision Res. 2002 Feb;42(4):401-15. IF: 2.0

Benedetto Falsini, A. L. Lyubarsky, J. Lem, J. Chen, A. Iannaccone, E. N. Pugh Jr.

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

48 Citazioni (Scopus)

Abstract

Two genetically engineered strains of mice were used to characterize murine cone function electroretinographically, without interference of rod-driven responses: (1) mice with a deletion of the gene for the rod transducin alpha-subunit (transducin alpha-/-), and (2) mice with rod arrestin deleted (arrestin -/-). In the first three months of age, both strains have a normal complement of rods and normal rod structure, but transducin alpha-/- mice have no rod-driven responses to light, while rod-driven activity of arrestin -/- mice can be suppressed by a single intense flash for hours. In response to intense flashes the electroretinograms of these strains of mice showed a readily identifiable, pure-cone a-wave of approximately 10 microV saturating amplitude. A 530 nm background that saturates rod responses of wild type mice was found to desensitize the b-wave responses of mice of both transgenic lines, whether the b-waves were driven by photons captured by M- or UV-cone pigments. The desensitizing effect of the 530 nm background on UV-pigment driven responses provides new evidence in support of the hypothesis of functional co-expression of the M-pigment in cones expressing primarily the UV-pigment.
Lingua originaleEnglish
pagine (da-a)401-415
Numero di pagine15
RivistaVision Research
Stato di pubblicazionePubblicato - 2002

Keywords

  • Electroretinogram
  • retinal degenerations

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