TY - JOUR
T1 - Foliar and Root Comparative Metabolomics and Phenolic Profiling of Micro-Tom Tomato (Solanum lycopersicum L.) Plants Associated with a Gene Expression Analysis in Response to Short Daily UV Treatments
AU - Mannucci, Alessia
AU - Santin, Marco
AU - Vanhaelewyn, Lucas
AU - Sciampagna, Maria Calogera
AU - Miras Moreno, Maria Begona
AU - Zhang, Leilei
AU - Lucini, Luigi
AU - Quartacci, Mike Frank
AU - Van Der Straeten, Dominique
AU - Castagna, Antonella
AU - Ranieri, Annamaria
PY - 2022
Y1 - 2022
N2 - Tomato (Solanum lycopersicum L.) is globally recognised as a high-value crop both for commercial profit and nutritional benefits. In contrast to the extensive data regarding the changes in the metabolism of tomato fruit exposed to UV radiation, less is known about the foliar and root metabolome. Using an untargeted metabolomic approach through UHPLC-ESI-QTOF-MS analysis, we detected thousands of metabolites in the leaves (3000) and roots (2800) of Micro-Tom tomato plants exposed to 11 days of short daily UV radiation, applied only on the aboveground organs. Multivariate statistical analysis, such as OPLS-DA and volcano, were performed to allow a better understanding of the modifications caused by the treatment. Based on the unexpected modulation to the secondary metabolism, especially the phenylpropanoid pathway, of which compounds were down and up accumulated respectively in leaves and roots of treated plants, a phenolic profiling was carried out for both organs. The phenolic profile was associated with a gene expression analysis to check the transcription trend of genes involved in the UVR8 signalling pathway and the early steps of the phenolic biosynthesis. The retention of the modifications at metabolic and phenolic levels was also investigated 3 days after the UV treatment, showing a prolonged effect on the modulation once the UV treatment had ceased.
AB - Tomato (Solanum lycopersicum L.) is globally recognised as a high-value crop both for commercial profit and nutritional benefits. In contrast to the extensive data regarding the changes in the metabolism of tomato fruit exposed to UV radiation, less is known about the foliar and root metabolome. Using an untargeted metabolomic approach through UHPLC-ESI-QTOF-MS analysis, we detected thousands of metabolites in the leaves (3000) and roots (2800) of Micro-Tom tomato plants exposed to 11 days of short daily UV radiation, applied only on the aboveground organs. Multivariate statistical analysis, such as OPLS-DA and volcano, were performed to allow a better understanding of the modifications caused by the treatment. Based on the unexpected modulation to the secondary metabolism, especially the phenylpropanoid pathway, of which compounds were down and up accumulated respectively in leaves and roots of treated plants, a phenolic profiling was carried out for both organs. The phenolic profile was associated with a gene expression analysis to check the transcription trend of genes involved in the UVR8 signalling pathway and the early steps of the phenolic biosynthesis. The retention of the modifications at metabolic and phenolic levels was also investigated 3 days after the UV treatment, showing a prolonged effect on the modulation once the UV treatment had ceased.
KW - UHPLC-ESI-QTOF-MS
KW - plant organs
KW - qRT-PCR
KW - secondary metabolism
KW - ultraviolet radiation
KW - untargeted metabolomics
KW - UHPLC-ESI-QTOF-MS
KW - plant organs
KW - qRT-PCR
KW - secondary metabolism
KW - ultraviolet radiation
KW - untargeted metabolomics
UR - http://hdl.handle.net/10807/232194
U2 - 10.3390/plants11141829
DO - 10.3390/plants11141829
M3 - Article
SN - 2223-7747
VL - 11
SP - 1829
EP - 1829
JO - Plants
JF - Plants
ER -