TY - JOUR
T1 - Ferritin nanovehicle for targeted delivery of cytochrome C to cancer cells
AU - Macone, Alberto
AU - Masciarelli, Silvia
AU - Palombarini, Federica
AU - Quaglio, Deborah
AU - Boffi, Alberto
AU - Trabuco, Matilde Cardoso
AU - Baiocco, Paola
AU - Fazi, Francesco
AU - Bonamore, Alessandra
PY - 2019
Y1 - 2019
N2 - In this work, we have exploited the unique properties of a chimeric archaeal-human ferritin to encapsulate, deliver and release cytochrome c and induce apoptosis in a myeloid leukemia cell line. The chimeric protein combines the versatility in 24-meric assembly and cargo incorporation capability of Archaeglobus fulgidus ferritin with specific binding of human H ferritin to CD71, the “heavy duty” carrier responsible for transferrin-iron uptake. Delivery of ferritin-encapsulated cytochrome C to the Acute Promyelocytic Leukemia (APL) NB4 cell line, highly resistant to transfection by conventional methods, was successfully achieved in vitro. The effective liberation of cytochrome C within the cytosolic environment, demonstrated by double fluorescent labelling, induced apoptosis in the cancer cells.
AB - In this work, we have exploited the unique properties of a chimeric archaeal-human ferritin to encapsulate, deliver and release cytochrome c and induce apoptosis in a myeloid leukemia cell line. The chimeric protein combines the versatility in 24-meric assembly and cargo incorporation capability of Archaeglobus fulgidus ferritin with specific binding of human H ferritin to CD71, the “heavy duty” carrier responsible for transferrin-iron uptake. Delivery of ferritin-encapsulated cytochrome C to the Acute Promyelocytic Leukemia (APL) NB4 cell line, highly resistant to transfection by conventional methods, was successfully achieved in vitro. The effective liberation of cytochrome C within the cytosolic environment, demonstrated by double fluorescent labelling, induced apoptosis in the cancer cells.
KW - Ferritin
KW - apoferritin
KW - Ferritin
KW - apoferritin
UR - http://hdl.handle.net/10807/147482
UR - http://www.nature.com/srep/index.html
U2 - 10.1038/s41598-019-48037-z
DO - 10.1038/s41598-019-48037-z
M3 - Article
SN - 2045-2322
VL - 9
SP - 11749-N/A
JO - Scientific Reports
JF - Scientific Reports
ER -