Ferritin nanovehicle for targeted delivery of cytochrome C to cancer cells

Silvia Masciarelli, Alberto Macone, Federica Palombarini, Deborah Quaglio, Alberto Boffi, Matilde Cardoso Trabuco, Paola Baiocco, Francesco Fazi, Alessandra Bonamore

Risultato della ricerca: Contributo in rivistaArticolo in rivista

16 Citazioni (Scopus)

Abstract

In this work, we have exploited the unique properties of a chimeric archaeal-human ferritin to encapsulate, deliver and release cytochrome c and induce apoptosis in a myeloid leukemia cell line. The chimeric protein combines the versatility in 24-meric assembly and cargo incorporation capability of Archaeglobus fulgidus ferritin with specific binding of human H ferritin to CD71, the “heavy duty” carrier responsible for transferrin-iron uptake. Delivery of ferritin-encapsulated cytochrome C to the Acute Promyelocytic Leukemia (APL) NB4 cell line, highly resistant to transfection by conventional methods, was successfully achieved in vitro. The effective liberation of cytochrome C within the cytosolic environment, demonstrated by double fluorescent labelling, induced apoptosis in the cancer cells.
Lingua originaleEnglish
pagine (da-a)11749-N/A
RivistaScientific Reports
Volume9
DOI
Stato di pubblicazionePubblicato - 2019

Keywords

  • Ferritin
  • apoferritin

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