Expression of the stem cell marker CD133 in recurrent glioblastoma and its value for prognosis

Nicola Montano, Francesco Pierconti, Ruggero De Maria Marchiano, Roberto Pallini, Tonia Cenci, Maurizio Martini, Luigi Maria Larocca, Lucia Ricci-Vitiani, Cristiana Mollinari, Mauro Biffoni

Risultato della ricerca: Contributo in rivistaArticolo in rivista

80 Citazioni (Scopus)


BACKGROUND: Experimental data suggest that glioblastoma cells expressing the stem cell marker CD133 play a major role in radiochemoresistance and tumor aggressiveness. To date, however, there is no clinical evidence that the fraction of CD133-positive cells in glioblastoma that recurs after radiochemotherapy may be relevant for prognosis. METHODS: The authors used immunohistochemistry to assess CD133 expression in 37 paired glioblastoma samples, including 1 primary tumor sample and 1 recurrent tumor sample, after patients received adjuvant radiochemotherapy. To assess the actual composition of the CD133-positive glioblastoma cell population, fluorescence-associated cell sorting (FACS) analysis was used to sort CD133-positive/CD45-negative cells that were assayed for tumor-specific chromosomal aberrations using interphase fluorescence in situ hybridization. To rule out endothelial precursor cells, CD133-positive fractions also were assayed with anti-CD34 by FACS. RESULTS: In recurrent glioblastomas, the percentage of CD133-positive cells was increased by 4.6-fold compared with the percentage in primary glioblastomas, although, in some tumors, it increased up to 10-fold and 20-fold. Unexpectedly, the increase in CD133 expression was associated significantly with longer survival after tumor recurrence. An analysis of tumor-specific chromosomal aberrations and in vivo studies revealed that the CD133-positive cell compartment of recurrent glioblastoma was composed of both cancer stem cells and nontumor neural stem cells. The latter cells represented from 20% to 60% of the CD133-positive cell population, and their relative percentage favorably affected the survival of patients with recurrent glioblastoma. Endothelial CD133-positive/CD34-positive precursors did not contribute to the CD133-positive cell population. CONCLUSIONS: The authors hypothesized that, similar to the phenomenon described in glioblastoma models, neural stem/progenitor cells that are recruited by the tumor from surrounding brain may exert an antitumorigenic effect. © 2010 American Cancer Society.
Lingua originaleEnglish
pagine (da-a)162-174
Numero di pagine13
Stato di pubblicazionePubblicato - 2011


  • AC133 Antigen
  • Adult
  • Aged
  • Antigens, CD
  • Biomarkers, Tumor
  • Brain Neoplasms
  • CD133
  • Cancer Research
  • Female
  • Glioblastoma
  • Glycoproteins
  • Humans
  • Male
  • Middle Aged
  • Neoplastic Stem Cells
  • Neural Stem Cells
  • Oncology
  • Peptides
  • Prognosis
  • Recurrence
  • cancer stem cells
  • glioblastoma
  • tumor recurrence


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