TY - JOUR
T1 - Expression of substance P, neurokinin 1 receptors(NK1) and neurokinin 3 receptors in the developing mouse retina and in the retina of NK1 knockout mice
AU - Catalani, Elisabetta
AU - Dal Monte, M
AU - Gangitano, Carlo
AU - Lucattelli, M
AU - Fineschi, S
AU - Bosco, L
AU - Bagnoli, P
AU - Casini, G.
PY - 2006
Y1 - 2006
N2 - Abstract—To complete a series of studies on the expression
of substance P and neurokinin receptors in mammalian retinas,
we investigated the occurrence of these molecules in
developing mouse retinas and in retinas of mice with genetic
deletion of the neurokinin 1 receptor, the preferred substance
P receptor. Using semi-quantitative reverse transcription–
polymerase chain reaction, we measured detectable levels of
the isoform of preprotachykinin A (a substance P precursor)
mRNA at postnatal day 4. Neurokinin 1 receptor and
neurokinin 3 receptor mRNAs were also detected at postnatal
day 4. While preprotachykinin A and neurokinin 1 receptor
mRNA levels significantly increased up to eye opening (postnatal
day 11), neurokinin 3 receptor mRNA levels remained
constant throughout development. Substance P, neurokinin
1 receptor and neurokinin 3 receptor immunoreactivities
were present at postnatal day 5. Substance P was in amacrine
cells, neurokinin 1 receptor in developing amacrine and bipolar
cells and neurokinin 3 receptor in OFF-type cone bipolar
cells. Interestingly, a transient increase in the density of
neurokinin 1 receptor immunoreactive processes was observed
at eye opening in lamina 3 of the inner plexiform layer,
suggesting a role of substance P and neurokinin 1 receptor in
this developmental phase. However, in neurokinin 1 receptor
knockout retinas, besides a significant increase of the
preprotachykinin A mRNA levels, no major changes were
detected: neurokinin 3 receptor mRNA levels as well as substance
P and neurokinin 3 receptor immunostainings were
similar to wild types. Together with previous studies, these
observations indicate that there are major differences in neurokinin
1 receptor expression patterns among developing mammalian retinas. The observations in neurokinin 1 receptor
knockout mice may not be applicable to rats or rabbits,
and substance P and neurokinin 1 receptor may play different
developmental roles in different species.
AB - Abstract—To complete a series of studies on the expression
of substance P and neurokinin receptors in mammalian retinas,
we investigated the occurrence of these molecules in
developing mouse retinas and in retinas of mice with genetic
deletion of the neurokinin 1 receptor, the preferred substance
P receptor. Using semi-quantitative reverse transcription–
polymerase chain reaction, we measured detectable levels of
the isoform of preprotachykinin A (a substance P precursor)
mRNA at postnatal day 4. Neurokinin 1 receptor and
neurokinin 3 receptor mRNAs were also detected at postnatal
day 4. While preprotachykinin A and neurokinin 1 receptor
mRNA levels significantly increased up to eye opening (postnatal
day 11), neurokinin 3 receptor mRNA levels remained
constant throughout development. Substance P, neurokinin
1 receptor and neurokinin 3 receptor immunoreactivities
were present at postnatal day 5. Substance P was in amacrine
cells, neurokinin 1 receptor in developing amacrine and bipolar
cells and neurokinin 3 receptor in OFF-type cone bipolar
cells. Interestingly, a transient increase in the density of
neurokinin 1 receptor immunoreactive processes was observed
at eye opening in lamina 3 of the inner plexiform layer,
suggesting a role of substance P and neurokinin 1 receptor in
this developmental phase. However, in neurokinin 1 receptor
knockout retinas, besides a significant increase of the
preprotachykinin A mRNA levels, no major changes were
detected: neurokinin 3 receptor mRNA levels as well as substance
P and neurokinin 3 receptor immunostainings were
similar to wild types. Together with previous studies, these
observations indicate that there are major differences in neurokinin
1 receptor expression patterns among developing mammalian retinas. The observations in neurokinin 1 receptor
knockout mice may not be applicable to rats or rabbits,
and substance P and neurokinin 1 receptor may play different
developmental roles in different species.
KW - amcrine cells
KW - bipolar cells
KW - inner plexiform layer
KW - neuropeptide receptors
KW - vesicular acetylcholine transporter
KW - amcrine cells
KW - bipolar cells
KW - inner plexiform layer
KW - neuropeptide receptors
KW - vesicular acetylcholine transporter
UR - http://hdl.handle.net/10807/17265
M3 - Article
SN - 0306-4522
VL - 138
SP - 487
EP - 499
JO - Neuroscience
JF - Neuroscience
ER -