Expression, localization and control of activin A release from human umbilical vein endothelial cells.

Rosanna Apa, Lavinia E. Borges, Enrrico Bloise, Cynthia Dela Cruz, Lauretta Massai, Pasquapina Ciarmela, Stefano Luisi, Filiberto M. Severi, Felice Petraglia, Fernando M. Reis

Risultato della ricerca: Contributo in rivistaArticolo in rivista

3 Citazioni (Scopus)

Abstract

Activin-A is a member of the TGFβ superfamily found in maternal and umbilical cord blood throughout gestation. We investigated whether human umbilical vein endothelial cells (HUVEC) express activin-A in vivo and tested the effects of vasoactive (endothelin-1), pro-inflammatory (interferon-γ, interleukin-8) and anti-inflammatory (dexamethasone, urocortin) factors on activin-A release by isolated HUVEC in vitro. Activin βA subunit protein and mRNA were strongly localized in the endothelial cells of umbilical veins and were also detectable in scattered cells of the cord connective tissue. Dimeric activin-A was detected in the HUVEC culture medium at picomolar concentrations. Activin-A release by HUVEC decreased after cell incubation with urocortin (p < 0.01), whereas no effect was observed with interleukin-8, interferon-γ, endothelin-1 or dexamethasone. In summary, activin-A is present in the human umbilical vein endothelium in vivo and is produced and released by isolated HUVEC. Activin-A secretion is inhibited in vitro by urocortin, a neuropeptide with predominantly anti-inflammatory action.
Lingua originaleEnglish
pagine (da-a)243-249
Numero di pagine7
RivistaGrowth Factors
Stato di pubblicazionePubblicato - 2015

Keywords

  • Activin A

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