Expression Cloning and Biochemical Characterizations of Recombinant Cyclophilin Proteins from Schistosoma mansoni

Francesca Bugli, A Khattab, E Vigneti, R Butler, D Cioli, Mq Klinkert

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

13 Citazioni (Scopus)

Abstract

Recombinant Schistosoma mansoni cyclophilin proteins of the A and the B subtypes (SmCYP A and B) were expressed in bacterial cells as histidine- and maltose-binding fusion proteins and also as nonfused proteins. In addition, S. mansoni CYPs were produced in Sf9 insect cells in their natural forms. Purified recombinant SmCYP B was found to possess a peptidyl-prolyl cis-trans isomerase (PPIase) activity, with a kcat/Km value of 8.2 x 10(5) M-1 s-1. The SmCYP B isoform is approximately two to three times more active than SmCYP A. SmCYP B-specific RNA appears to be more abundant in adult schistosomes than SmCYP A RNA in Northern blots. These results support the conclusion that SmCYP B represents the major schistosomal CYP. The PPIase-associated activity of both CYPs was inhibitable by the immunosuppressive drug cyclosporin A (CsA). We attempt to explain differences in PPIase activities and in CsA inhibition by examining models of the two CYPs complexed to CsA
Lingua originaleEnglish
pagine (da-a)240-246
Numero di pagine7
RivistaPROTEIN EXPRESSION AND PURIFICATION
Volume12
DOI
Stato di pubblicazionePubblicato - 1998

Keywords

  • Cyclosporin A
  • Recombinant Cyclophilin

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