TY - JOUR
T1 - Exploring the brain tissue proteome of TgCRND8 Alzheimer's Disease model
mice under B vitamin deficient diet induced hyperhomocysteinemia by
LC-MS top-down platform
AU - Delfino, Daniela
AU - Rossetti, Diana Valeria
AU - Martelli, Claudia
AU - Inserra, Ilaria
AU - Vincenzoni, Federica
AU - Castagnola, Massimo
AU - Urbani, Andrea
AU - Scarpa, Sigfrido
AU - Fuso, Andrea
AU - Fuso, Paola
AU - Cavallaro, Rosaria A.
AU - Desiderio, Claudia
PY - 2019
Y1 - 2019
N2 - The multifactorial nature of Late Onset Alzheimer's Disease (LOAD), the AD form of major relevance on epidemiological and social aspects, has driven the original investigation by LC-MS and top-down proteomics approach of the protein repertoire of the brain tissue of TgCRND8 model mice fed with a diet deficient in B vitamins. The analysis of the acid-soluble fraction of brain tissue homogenates identified a list of proteins and peptides, proteoforms and PTMs. In order to disclose possible modulations, their relative quantification in wild type and AD model mice under both B vitamin deficient and control diets was performed. The levels of metallothionein III, guanine nucleotide-binding protein G(I)/G(S)/G(0) subunit gamma-2 and brain acid soluble protein 1 showed statistically significant alterations depending on genotype, diet or both effects, respectively. Particularly, metallothionein III exhibited increased levels in TgCRND8 mice under B vitamin deficient diet with respect to wild type mice under both diets. Brain acid soluble protein 1 showed the opposite, revealing decreased levels in all diet groups of AD model mice with respect to wild type mice in control diet. Lower levels of brain acid soluble protein 1 were also observed in wild type mice under deficiency of B vitamins. These results, besides contributing to increase the knowledge of AD at molecular level, give new suggestions for deeply investigating metallothionein III and brain acid soluble protein 1 in AD.
AB - The multifactorial nature of Late Onset Alzheimer's Disease (LOAD), the AD form of major relevance on epidemiological and social aspects, has driven the original investigation by LC-MS and top-down proteomics approach of the protein repertoire of the brain tissue of TgCRND8 model mice fed with a diet deficient in B vitamins. The analysis of the acid-soluble fraction of brain tissue homogenates identified a list of proteins and peptides, proteoforms and PTMs. In order to disclose possible modulations, their relative quantification in wild type and AD model mice under both B vitamin deficient and control diets was performed. The levels of metallothionein III, guanine nucleotide-binding protein G(I)/G(S)/G(0) subunit gamma-2 and brain acid soluble protein 1 showed statistically significant alterations depending on genotype, diet or both effects, respectively. Particularly, metallothionein III exhibited increased levels in TgCRND8 mice under B vitamin deficient diet with respect to wild type mice under both diets. Brain acid soluble protein 1 showed the opposite, revealing decreased levels in all diet groups of AD model mice with respect to wild type mice in control diet. Lower levels of brain acid soluble protein 1 were also observed in wild type mice under deficiency of B vitamins. These results, besides contributing to increase the knowledge of AD at molecular level, give new suggestions for deeply investigating metallothionein III and brain acid soluble protein 1 in AD.
KW - Alzheimer's Disease
KW - TgCRND8 mice
KW - Alzheimer's Disease
KW - TgCRND8 mice
UR - http://hdl.handle.net/10807/153380
U2 - 10.1016/j.jchromb.2019.06.005
DO - 10.1016/j.jchromb.2019.06.005
M3 - Article
SN - 1570-0232
VL - 1124
SP - 165
EP - 172
JO - JOURNAL OF CHROMATOGRAPHY. B
JF - JOURNAL OF CHROMATOGRAPHY. B
ER -