We have investigated the mechanical properties of the zona pellucida ZP , a multilayer glycoprotein coat that surrounds mammalian eggs, using atomic force spectroscopy. The response of the membrane to mechanical stress has been reconstructed using a modified Hertz model. The transition from elastic behavior, which occurs when low stress forces are applied characterized by a Young s modulus E= 22 5 kPa , toward plastic behavior is observed. The critical indentation necessary to induce plastic deformations occurs at yield= 550 50 nm. This high critical value, corresponding to two ZP layers, well supports the noncovalent long lifetimes of interactions that take place between constituent glycoproteins.
|Numero di pagine||3|
|Rivista||Applied Physics Letters|
|Stato di pubblicazione||Pubblicato - 2009|
- Atomic Force Spectroscopy
- Zona Pellucida