Evaluation of the New NucliSENS EasyQ KPC test for rapid detection of Klebsiella pneumoniae carbapenemase genes (blaKPC)

Teresa Spanu; Tiziana D'Inzeo; Ivana Palucci; Maurizio Sanguinetti; Barbara Fiori Alfarano; Giulia Canu; Serena Campoli; Mario Tumbarello; T Giani; Gm Rossolini

doi:10.1128/JCM.00284-12

Evaluation of the New NucliSENS EasyQ KPC test for rapid detection of Klebsiella pneumoniae carbapenemase genes (blaKPC)

Teresa Spanu, Tiziana D'Inzeo, Ivana Palucci, Maurizio Sanguinetti, Barbara Fiori Alfarano, Giulia Canu, Serena Campoli, Mario Tumbarello, T Giani, Gm Rossolini

Risultato della ricerca: Contributo in rivista › Articolo in rivista › peer review

32 Citazioni (Scopus)

Abstract

KPC-type carbapenemases are emerging in Klebsiella pneumoniae and other Gram-negative pathogens worldwide. Rapid and sensitive detection of these resistance determinants has become relevant to clinical management and infection control. We evaluated the bioMérieux EasyQ real-time PCR assay for bla(KPC) detection with 300 members of the Enterobacteriaceae, including 29 control strains producing known carbapenemases and 271 nonreplicate clinical isolates. The EasyQ assay correctly detected all of the 111 isolates harboring bla(KPC) genes, with no false positives, and results were available within 2 h.

Lingua originale	English
pagine (da-a)	2783-2785
Numero di pagine	3
Rivista	Journal of Clinical Microbiology
Volume	50
DOI	https://doi.org/10.1128/JCM.00284-12
Stato di pubblicazione	Pubblicato - 2012

Keywords

Bacterial Proteins
Bacteriological Techniques
Enterobacteriaceae
Enterobacteriaceae Infections
Humans
Molecular Diagnostic Techniques
Real-Time Polymerase Chain Reaction
Sensitivity and Specificity
Time Factors
beta-Lactam Resistance
beta-Lactamases

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abstract = "KPC-type carbapenemases are emerging in Klebsiella pneumoniae and other Gram-negative pathogens worldwide. Rapid and sensitive detection of these resistance determinants has become relevant to clinical management and infection control. We evaluated the bioM{\'e}rieux EasyQ real-time PCR assay for bla(KPC) detection with 300 members of the Enterobacteriaceae, including 29 control strains producing known carbapenemases and 271 nonreplicate clinical isolates. The EasyQ assay correctly detected all of the 111 isolates harboring bla(KPC) genes, with no false positives, and results were available within 2 h.",

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AU - Spanu, Teresa

AU - D'Inzeo, Tiziana

AU - Palucci, Ivana

AU - Sanguinetti, Maurizio

AU - Fiori Alfarano, Barbara

AU - Canu, Giulia

AU - Campoli, Serena

AU - Tumbarello, Mario

AU - Giani, T

AU - Rossolini, Gm

PY - 2012

Y1 - 2012

N2 - KPC-type carbapenemases are emerging in Klebsiella pneumoniae and other Gram-negative pathogens worldwide. Rapid and sensitive detection of these resistance determinants has become relevant to clinical management and infection control. We evaluated the bioMérieux EasyQ real-time PCR assay for bla(KPC) detection with 300 members of the Enterobacteriaceae, including 29 control strains producing known carbapenemases and 271 nonreplicate clinical isolates. The EasyQ assay correctly detected all of the 111 isolates harboring bla(KPC) genes, with no false positives, and results were available within 2 h.

AB - KPC-type carbapenemases are emerging in Klebsiella pneumoniae and other Gram-negative pathogens worldwide. Rapid and sensitive detection of these resistance determinants has become relevant to clinical management and infection control. We evaluated the bioMérieux EasyQ real-time PCR assay for bla(KPC) detection with 300 members of the Enterobacteriaceae, including 29 control strains producing known carbapenemases and 271 nonreplicate clinical isolates. The EasyQ assay correctly detected all of the 111 isolates harboring bla(KPC) genes, with no false positives, and results were available within 2 h.

KW - Bacterial Proteins

KW - Bacteriological Techniques

KW - Enterobacteriaceae

KW - Enterobacteriaceae Infections

KW - Humans

KW - Molecular Diagnostic Techniques

KW - Real-Time Polymerase Chain Reaction

KW - Sensitivity and Specificity

KW - Time Factors

KW - beta-Lactam Resistance

KW - beta-Lactamases

KW - Bacterial Proteins

KW - Bacteriological Techniques

KW - Enterobacteriaceae

KW - Enterobacteriaceae Infections

KW - Humans

KW - Molecular Diagnostic Techniques

KW - Real-Time Polymerase Chain Reaction

KW - Sensitivity and Specificity

KW - Time Factors

KW - beta-Lactam Resistance

KW - beta-Lactamases

UR - http://hdl.handle.net/10807/40694

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DO - 10.1128/JCM.00284-12

M3 - Article

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SP - 2783

EP - 2785

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

ER -

Evaluation of the New NucliSENS EasyQ KPC test for rapid detection of Klebsiella pneumoniae carbapenemase genes (blaKPC)

Abstract

Keywords

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