TY - JOUR
T1 - Evaluation of the New NucliSENS EasyQ KPC test for rapid detection of Klebsiella pneumoniae carbapenemase genes (blaKPC)
AU - Spanu, Teresa
AU - Fiori, Barbara
AU - D'Inzeo, Tiziana
AU - Canu, Giulia
AU - Campoli, Serena
AU - Giani, Tommaso
AU - Palucci, Ivana
AU - Tumbarello, Mario
AU - Sanguinetti, Maurizio
AU - Rossolini, Gian Maria
PY - 2012
Y1 - 2012
N2 - KPC-type carbapenemases are emerging in Klebsiella pneumoniae and other Gram-negative pathogens worldwide. Rapid and sensitive detection of these resistance determinants has become relevant to clinical management and infection control. We evaluated the bioMérieux EasyQ real-time PCR assay for bla(KPC) detection with 300 members of the Enterobacteriaceae, including 29 control strains producing known carbapenemases and 271 nonreplicate clinical isolates. The EasyQ assay correctly detected all of the 111 isolates harboring bla(KPC) genes, with no false positives, and results were available within 2 h.
AB - KPC-type carbapenemases are emerging in Klebsiella pneumoniae and other Gram-negative pathogens worldwide. Rapid and sensitive detection of these resistance determinants has become relevant to clinical management and infection control. We evaluated the bioMérieux EasyQ real-time PCR assay for bla(KPC) detection with 300 members of the Enterobacteriaceae, including 29 control strains producing known carbapenemases and 271 nonreplicate clinical isolates. The EasyQ assay correctly detected all of the 111 isolates harboring bla(KPC) genes, with no false positives, and results were available within 2 h.
KW - Bacterial Proteins
KW - Bacteriological Techniques
KW - Enterobacteriaceae
KW - Enterobacteriaceae Infections
KW - Humans
KW - Molecular Diagnostic Techniques
KW - Real-Time Polymerase Chain Reaction
KW - Sensitivity and Specificity
KW - Time Factors
KW - beta-Lactam Resistance
KW - beta-Lactamases
KW - Bacterial Proteins
KW - Bacteriological Techniques
KW - Enterobacteriaceae
KW - Enterobacteriaceae Infections
KW - Humans
KW - Molecular Diagnostic Techniques
KW - Real-Time Polymerase Chain Reaction
KW - Sensitivity and Specificity
KW - Time Factors
KW - beta-Lactam Resistance
KW - beta-Lactamases
UR - http://hdl.handle.net/10807/40694
U2 - 10.1128/JCM.00284-12
DO - 10.1128/JCM.00284-12
M3 - Article
SN - 1098-660X
VL - 50
SP - 2783
EP - 2785
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
ER -