TY - JOUR
T1 - Evaluation of the Abbott LCx Mycobacterium tuberculosis assay in comparison with culture methods in selected Italian patients
AU - Fadda, Giovanni
AU - Ardito, Fausta
AU - Sanguinetti, Maurizio
AU - Posteraro, Brunella
AU - Ortona, Luigi
AU - Chezzi, C.
AU - Polonelli, L.
AU - Dettori, G.
AU - Conti, S.
AU - Fanti, F.
AU - Galli, C.
PY - 1998
Y1 - 1998
N2 - A ligase chain reaction (LCR) DNA amplification method for the molecular diagnosis of Mycobacterium tuberculosis (Abbott LCx MTB) was evaluated in comparison with solid and liquid phase culture on 622 selected samples collected in two large Italian hospitals, of which 310 obtained from HIV-1 positive patients and 312 from HIV-negative individuals. The overall prevalence of mycobacteria by culture was 22% (137/622), and the apparent sensitivity and specificity of LCx vs. culture were 87.6% and 98.2%. Of the 26 culture positive/LCx negative samples, 22 were positive for MOTT and 4 for M. tuberculosis. All 9 samples positive by LCx and negative by culture were classified as true positive by clinical criteria. The final values of sensitivity, specificity, positive predictive value and negative predictive value for LCx rose to 96.8%, 100%, 100% and 99.2%, respectively. The adjusted sensitivity of culture methods was 89.5% for solid phase and 92.7% for Bactec. In view of the high sensitivity on both smear-positive (100%) and smear-negative (92.4%) samples and of the high negative predictive value, the LCR-based amplification method appears suitable as a routine screening method for the rapid diagnosis of M. tuberculosis in high-risk patients.
AB - A ligase chain reaction (LCR) DNA amplification method for the molecular diagnosis of Mycobacterium tuberculosis (Abbott LCx MTB) was evaluated in comparison with solid and liquid phase culture on 622 selected samples collected in two large Italian hospitals, of which 310 obtained from HIV-1 positive patients and 312 from HIV-negative individuals. The overall prevalence of mycobacteria by culture was 22% (137/622), and the apparent sensitivity and specificity of LCx vs. culture were 87.6% and 98.2%. Of the 26 culture positive/LCx negative samples, 22 were positive for MOTT and 4 for M. tuberculosis. All 9 samples positive by LCx and negative by culture were classified as true positive by clinical criteria. The final values of sensitivity, specificity, positive predictive value and negative predictive value for LCx rose to 96.8%, 100%, 100% and 99.2%, respectively. The adjusted sensitivity of culture methods was 89.5% for solid phase and 92.7% for Bactec. In view of the high sensitivity on both smear-positive (100%) and smear-negative (92.4%) samples and of the high negative predictive value, the LCR-based amplification method appears suitable as a routine screening method for the rapid diagnosis of M. tuberculosis in high-risk patients.
KW - DNA, Bacterial
KW - Evaluation Studies as Topic
KW - Humans
KW - Italy
KW - Microbiological Techniques
KW - Mycobacterium tuberculosis
KW - Reagent Kits, Diagnostic
KW - Sensitivity and Specificity
KW - Tuberculosis
KW - DNA, Bacterial
KW - Evaluation Studies as Topic
KW - Humans
KW - Italy
KW - Microbiological Techniques
KW - Mycobacterium tuberculosis
KW - Reagent Kits, Diagnostic
KW - Sensitivity and Specificity
KW - Tuberculosis
UR - http://hdl.handle.net/10807/11407
M3 - Article
SN - 1121-7138
VL - 21
SP - 97
EP - 103
JO - New Microbiologica
JF - New Microbiologica
ER -