Evaluation of mass spectrometry detection of pan-fungal serum disaccharide (MS-DS) for the diagnosis of invasive fungal infections: results from a collaborative study involving six European clinical centres

Maurizio Sanguinetti, Elena De Carolis, Marjorie Cornu, Boualem Sendid, Alexandre Mery, Nadine François, Mikulska Malgorzata, Valérie Letscher-Bru, Lauro Damonti, Marie Titecat, Pierre-Yves Bochud, Alexandre Alanio, Claudio Viscoli, Raoul Herbrecht, Yann Guerardel, Daniel Poulaina

Risultato della ricerca: Contributo in rivistaArticolo in rivista

6 Citazioni (Scopus)

Abstract

Background A mass spectrometry (MS) method that detects a serum disaccharide (MS-DS) was recently described for the diagnosis of invasive fungal infections (IFI). We carried out a European collaborative study to evaluate this assay.Methods Patients with IFI were selected according to the availability of sera obtained around the time that IFI was documented: invasive candidiasis (IC; n=26 patients), invasive aspergillosis (IA; n=19), mucormycosis (MM; n=23). Control sera originated from 20 neutropenic patients and 20 patients with bacteraemia. MS-DS was carried out blind to IFI diagnosis. Diagnosis of IC and IA was confirmed by detection of (1,3)-β-D-glucan (BDG), mannan (Man) and galactomannan (GM), respectively. MM was detected by q-PCR.Results All tests discriminated IC sera from controls with bacteraemia (P≤0.0009). MS-DS sensitivity and specificity were 51% and 87%, respectively. MS-DS complemented the high specificity of Man monitoring. All tests discriminated IA sera from neutropenic controls (P≤0.0009). MS-DS sensitivity and specificity were 64% and 90%, respectively. Only 13/36 MM sera were concordant by MS-DS and q-PCR (six positive and seven negative); 14 were positive by MS-DS alone. q-PCR and MS-DS made a similar contribution to the diagnosis of MM. In patients undergoing long-term monitoring, persistent MS-DS circulation was observed whereas DNA was only detected for a short period after initiation of treatment.Conclusions MS-DS has an important role to play in the early diagnosis of IFI. Its pan fungal nature and complementarity with other tests may justify its use in the management of IFI.
Lingua originaleEnglish
pagine (da-a)N/A-N/A
RivistaJournal of Clinical Microbiology
DOI
Stato di pubblicazionePubblicato - 2019

Keywords

  • beta-glucan

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