Abstract
Previous studies had indicated an active role of bovine forestomachs in the response to digestive disorders as well as
to inflammatory and infectious processes in the gastro-intestinal (GI) tract and elsewhere. We investigated the
potential of bovine forestomachs to receive, amplify and produce mediators of the innate immune response. Indeed,
we detected the expression of TIR8 and other receptors and cytokines of the innate immune response, such as Tolllike
receptor (TLR)4, interleukin (IL)-1beta, IL-10 and Caspase-1 in the forestomach walls of healthy cows. Their
presence suggests an active role of forestomachs in inflammatory disorders of the GI tract and other body
compartments. We confirmed and further characterized the presence of leukocytes and cytokines in the rumen fluids.
In particular, T and B lymphocytes and myeloid lineage cells were detected in the ruminal content of both rumenfistulated
heifers and diseased cows. Also, leukocyte infiltration was modulated by feeding disorders: an acidogenic
diet based on daily supplements of maize was shown to inhibit leukocyte accumulation, as opposed to a control, haybased
diet, with or without a soy flour (protein) supplement. A survey of 11 dairy farms indicated that low-stress diets
and management were correlated with low levels of CD45 gene expression and B cell infiltration in rumen fluids, such
as in the “control farm”. In other farms, the rumen fluids of dairy cows showed increased levels of CD45 gene
expression and numbers of leukocytes of different lineages (B-cells, T-cells, myeloid cells). This latter pattern of
infiltration was correlated with an increased prevalence of inflammatory markers (positive and negative acute phase
responses). On the whole, our results indicate that bovine forestomachs can receive and amplify signals for the
immune cells infiltrating the rumen content and oral lymphoid tissues. Owing to the above, the examination of the
rumen fluid could be a novel and useful diagnostic tool to support the clinical inspections of cattle herds.
Lingua originale | English |
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Titolo della pubblicazione ospite | 10th International Veterinary Immunology Symposium - IVIS 2013 |
Pagine | 187 |
Numero di pagine | 1 |
Stato di pubblicazione | Pubblicato - 2013 |
Evento | 10th International Veterinary Immunology Symposium - IVIS 2013 - Milano Durata: 28 ago 2013 → 1 set 2013 |
Convegno
Convegno | 10th International Veterinary Immunology Symposium - IVIS 2013 |
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Città | Milano |
Periodo | 28/8/13 → 1/9/13 |
Keywords
- bovine
- control farm