Evaluation of a nonradiometric system (BACTEC 9000 MB) for detection of mycobacteria in human clinical samples

Maurizio Sanguinetti, Giovanni Delogu, Giovanni Fadda, Fausta Ardito, Aldo Nacci, S Zanetti, L Sechi, P Molicotti, Mp Pinna

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27 Citazioni (Scopus)

Abstract

This study was carried out to evaluate the rate of recovery and time required for detection of mycobacteria from pulmonary and extrapulmonary human clinical samples, by using a fluorescence-quenching-based oxygen sensor (BACTEC 9000 MB; Becton Dickinson Microbiology Systems, Sparks, Md.). The results were compared with those obtained by microscopy, conventional culture in Lowenstein-Jensen (LJ) medium, and a BACTEC radiometric system (BACTEC 460 TB; Becton Dickinson). Of the 779 clinical samples processed, 364 from pulmonary sites and 415 from extrapulmonary sites, 62 (7.9%) were positive for mycobacterial isolates; of the positive samples, 59 (95.1%) were detected with the fluorescent BACTEC 9000 MB system, 57 (91.9%) were detected with the radiometric system (BACTEC 460 TB), and 43 (69.3%) were detected with LJ conventional culture. The mean times to detection of all mycobacteria with BACTEC 9000 MB and BACTEC 460 TB were similar (10.3 and 10.0 days, respectively). The results obtained indicate that the nonradiometric BACTEC (BACTEC 9000 MB) system is as efficient as Bactec 460 TB and significantly more efficient than LJ for the rapid recovery of mycobacteria from both pulmonary and extrapulmonary clinical specimens. Though the BACTEC 9000 MB system is recommended for respiratory specimens, we demonstrated that it can be successfully used also for recovery of mycobacteria from clinical specimens from various extrapulmonary sites.
Lingua originaleEnglish
pagine (da-a)2072-2075
Numero di pagine4
RivistaJournal of Clinical Microbiology
Volume35
Stato di pubblicazionePubblicato - 1997

Keywords

  • Bacteriological Techniques
  • Cerebrospinal Fluid
  • Evaluation Studies as Topic
  • False Positive Reactions
  • Feces
  • Humans
  • Mycobacterium
  • Sputum
  • Time Factors
  • Urine

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