TY - JOUR
T1 - Erythrocyte membrane fluidity: A novel biomarker of residual cardiovascular risk in type 2 diabetes
AU - Bianchetti, Giada
AU - Cefalo, Chiara Maria Assunta
AU - Ferreri, Carla
AU - Sansone, Anna
AU - Vitale, Marilena
AU - Serantoni, Cassandra
AU - Abeltino, Alessio
AU - Mezza, Teresa
AU - Ferraro, Pietro Manuel
AU - De Spirito, Marco
AU - Riccardi, Gabriele
AU - Giaccari, Andrea
AU - Maulucci, Giuseppe
PY - 2024
Y1 - 2024
N2 - Aims: Improving the composition of circulating fatty acids (FA) leads to a reduction in cardiovascular diseases (CVD) in high-risk individuals. The membrane fluidity of red blood cells (RBC), which reflects circulating FA status, may be a valid biomarker of cardiovascular (CV) risk in type 2 diabetes (T2D). Methods: Red blood cell membrane fluidity, quantified as general polarization (GP), was assessed in 234 subjects with T2D, 86 with prior major CVD. Based on GP distribution, a cut-off of.445 was used to divide the study cohort into two groups: the first with higher GP, called GEL, and the second, defined as lower GP (LGP). Lipidomic analysis was performed to evaluate FA composition of RBC membranes. Results: Although with comparable CV risk factors, the LGP group had a greater percentage of patients with major CVD than the GEL group (40% vs 24%, respectively, p <.05). Moreover, in a logistic regression analysis, a lower GP value was independently associated with the presence of macrovascular complications. Lipidomic analysis showed a clear shift of LGP membranes towards a pro-inflammatory condition due to higher content of arachidonic acid and increased omega 6/omega 3 index. Conclusions: Increased membrane fluidity is associated with a higher CV risk in subjects with T2D. If confirmed in prospective studies, membrane fluidity could be a new biomarker for residual CV risk assessment in T2D.
AB - Aims: Improving the composition of circulating fatty acids (FA) leads to a reduction in cardiovascular diseases (CVD) in high-risk individuals. The membrane fluidity of red blood cells (RBC), which reflects circulating FA status, may be a valid biomarker of cardiovascular (CV) risk in type 2 diabetes (T2D). Methods: Red blood cell membrane fluidity, quantified as general polarization (GP), was assessed in 234 subjects with T2D, 86 with prior major CVD. Based on GP distribution, a cut-off of.445 was used to divide the study cohort into two groups: the first with higher GP, called GEL, and the second, defined as lower GP (LGP). Lipidomic analysis was performed to evaluate FA composition of RBC membranes. Results: Although with comparable CV risk factors, the LGP group had a greater percentage of patients with major CVD than the GEL group (40% vs 24%, respectively, p <.05). Moreover, in a logistic regression analysis, a lower GP value was independently associated with the presence of macrovascular complications. Lipidomic analysis showed a clear shift of LGP membranes towards a pro-inflammatory condition due to higher content of arachidonic acid and increased omega 6/omega 3 index. Conclusions: Increased membrane fluidity is associated with a higher CV risk in subjects with T2D. If confirmed in prospective studies, membrane fluidity could be a new biomarker for residual CV risk assessment in T2D.
KW - cardiovascular disease
KW - cardiovascular risk assessment
KW - fatty acids
KW - fluorescence microscopy
KW - machine-learning
KW - membrane fluidity
KW - type 2 diabetes
KW - cardiovascular disease
KW - cardiovascular risk assessment
KW - fatty acids
KW - fluorescence microscopy
KW - machine-learning
KW - membrane fluidity
KW - type 2 diabetes
UR - http://hdl.handle.net/10807/262622
U2 - 10.1111/eci.14121
DO - 10.1111/eci.14121
M3 - Article
SN - 0014-2972
VL - 54
SP - N/A-N/A
JO - European Journal of Clinical Investigation
JF - European Journal of Clinical Investigation
ER -