TY - JOUR
T1 - Erythrocyte glutathione transferase: A new biomarker in chronic
kidney diseases
AU - Calla', Cinzia Anna Maria
AU - Dessì, Mariarita
AU - Noce, Annalisa
AU - Fabrini, R
AU - Bocedi, A
AU - Fucci, A
AU - Pastore, Anna
AU - Manca Di Villahermosa, S
AU - Ricci, G.
PY - 2011
Y1 - 2011
N2 - Background: Glutathione transferases (GSTs) are enzymes involved
in the cell detoxification. Increased levels of GST expression
have been observed in tissues exposed to contaminants and in human
erythrocytes of uremic patients under chronic hemodialysis (HD). A
previous study reported that no difference in the erythrocyte GST
expression has been detected in patients under conservative therapy.
Aim of our study was to compare erythrocyte GST (e-GST) activity in
chronic kidney disease (CDK), in chronic hemodialysis (HD) and in
healthy subjects.
Methods: A total of 72 CKD patients under conservative therapy,
62 HD patients and 80 healthy subjects were enrolled. CKD patients
were divided in four groups according to the National Kidney
Foundation Dialysis Outcomes Quality Initiative (NK-DOQI). Erythrocyte
GST activity was assayed by means of a spectrophotometer
procedure at 340 nm on haemolysed whole blood adapted to an
automated Modular P800 apparatus (Roche).
Results: The e-GST activity was enhanced in HD patients (10.24±
2.74 U/g Hb) when compared to healthy subjects (5.8±1.8 U/g Hb).
Contrary to previous reports, a significant increase of e-GST activity
related to CKD stage was also observed in pre-dialysis patients (7.4±
2.43 U/g Hb, 8.13±4.55 U/g Hb, 9.46±2.49 U/g Hb, and 12.35±
2.74 U/g Hb in stages I to IV). Interestingly, e-GST activity in stage IV
patients is higher than in HD subjects. No correlation has been found
between e-GST activity and levels of classical systemic inflammation
markers.
Conclusion: The present findings suggest that e-GST could be a
good marker for toxin exposition. The e-GST activity could be a
new biomarker useful to substitute or to be complementary to the
time.
AB - Background: Glutathione transferases (GSTs) are enzymes involved
in the cell detoxification. Increased levels of GST expression
have been observed in tissues exposed to contaminants and in human
erythrocytes of uremic patients under chronic hemodialysis (HD). A
previous study reported that no difference in the erythrocyte GST
expression has been detected in patients under conservative therapy.
Aim of our study was to compare erythrocyte GST (e-GST) activity in
chronic kidney disease (CDK), in chronic hemodialysis (HD) and in
healthy subjects.
Methods: A total of 72 CKD patients under conservative therapy,
62 HD patients and 80 healthy subjects were enrolled. CKD patients
were divided in four groups according to the National Kidney
Foundation Dialysis Outcomes Quality Initiative (NK-DOQI). Erythrocyte
GST activity was assayed by means of a spectrophotometer
procedure at 340 nm on haemolysed whole blood adapted to an
automated Modular P800 apparatus (Roche).
Results: The e-GST activity was enhanced in HD patients (10.24±
2.74 U/g Hb) when compared to healthy subjects (5.8±1.8 U/g Hb).
Contrary to previous reports, a significant increase of e-GST activity
related to CKD stage was also observed in pre-dialysis patients (7.4±
2.43 U/g Hb, 8.13±4.55 U/g Hb, 9.46±2.49 U/g Hb, and 12.35±
2.74 U/g Hb in stages I to IV). Interestingly, e-GST activity in stage IV
patients is higher than in HD subjects. No correlation has been found
between e-GST activity and levels of classical systemic inflammation
markers.
Conclusion: The present findings suggest that e-GST could be a
good marker for toxin exposition. The e-GST activity could be a
new biomarker useful to substitute or to be complementary to the
time.
KW - glutathione
KW - glutathione
UR - http://hdl.handle.net/10807/40565
U2 - 10.1007/s00726-011-1085-x
DO - 10.1007/s00726-011-1085-x
M3 - Conference article
SN - 0009-9120
SP - 54
EP - 54
JO - Clinical Biochemistry
JF - Clinical Biochemistry
T2 - Congress
Y2 - 1 March 2011 through 3 March 2011
ER -