Abstract
A new procedure for the encapsulation of non-diffusible drugs into human erythrocytes was developed. With as little as 50 ml of blood and by using a new apparatus, it was possible to encapsulate a variety of biologically active compounds into erythrocytes in 2 h at room temperature and under blood-banking conditions, The process, which is based on two sequential hypotonic dilutions of washed red cells followed by concentration with a haemofilter and resealing of red cells, allows a 35-50% cell recovery and approx, 30% encapsulation of added drugs. The resulting processed erythrocytes have a normal survival in vivo and can be modified further, with the same apparatus, to; Increase their recognition by tissue macrophages to perform as a drug-targeting system. The new equipment designed and built for this procedure was named 'Red Cell Loader'.
Lingua originale | English |
---|---|
pagine (da-a) | 1-6 |
Numero di pagine | 6 |
Rivista | Biotechnology and Applied Biochemistry |
Volume | 28 |
Stato di pubblicazione | Pubblicato - 1998 |
Keywords
- drug delivery
- erytrocyte