Erythrocyte engineering for drug delivery and targeting

Marcello D'Ascenzo, Mauro Magnani, Luigia Rossi, Ivo Panzani, Leonardo Bigi, Andrea Zanella

Risultato della ricerca: Contributo in rivistaArticolo in rivista

83 Citazioni (Scopus)

Abstract

A new procedure for the encapsulation of non-diffusible drugs into human erythrocytes was developed. With as little as 50 ml of blood and by using a new apparatus, it was possible to encapsulate a variety of biologically active compounds into erythrocytes in 2 h at room temperature and under blood-banking conditions, The process, which is based on two sequential hypotonic dilutions of washed red cells followed by concentration with a haemofilter and resealing of red cells, allows a 35-50% cell recovery and approx, 30% encapsulation of added drugs. The resulting processed erythrocytes have a normal survival in vivo and can be modified further, with the same apparatus, to; Increase their recognition by tissue macrophages to perform as a drug-targeting system. The new equipment designed and built for this procedure was named 'Red Cell Loader'.
Lingua originaleEnglish
pagine (da-a)1-6
Numero di pagine6
RivistaBiotechnology and Applied Biochemistry
Volume28
Stato di pubblicazionePubblicato - 1998

Keywords

  • drug delivery
  • erytrocyte

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