Enterococcal Rgg-Like Regulator ElrR Activates Expression of the elrA Operon

Romain Dumoulin, Naima Cortes-Perez, Stephane Gaubert, Philippe Duhutrel, Sophie Brinster, Riccardo Torelli, Maurizio Sanguinetti, Brunella Posteraro, Francis Repoila, Pascale Serror

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The Enterococcus faecalis leucine-rich protein ElrA promotes virulence by stimulating bacterial persistence in macrophages and production of the interleukin-6 (IL-6) cytokine. The ElrA protein is encoded within an operon that is poorly expressed under laboratory conditions but induced in vivo. In this study, we identify ef2687 (renamed elrR), which encodes a member of the Rgg (regulator gene for glucosyltransferase) family of putative regulatory proteins. Using quantitative reverse transcription-PCR, translational lacZ fusions, and electrophoretic mobility shift assays, we demonstrate that ElrR positively regulates expression of elrA. These results correlate with the attenuated virulence of the ΔelrR strain in a mouse peritonitis model. Virulence of simple and double elrR and elrA deletion mutants also suggests a remaining ElrR-independent expression of elrA in vivo and additional virulence-related genes controlled by ElrR.
Lingua originaleEnglish
pagine (da-a)3073-3083
Numero di pagine11
RivistaJournal of Bacteriology
Stato di pubblicazionePubblicato - 2013


  • Enterococcus faecalis


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