Engineering human monoclonal antibody fragments: a recombinant enzyme-linked Fab

Rosaria Santangelo, Paola Plaisant

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

6 Citazioni (Scopus)

Abstract

A new plasmid vector, pCRP, allowing the expression of human recombinant monoclonal antibody Fab fragments fused with a bacterial acid phosphatase has been constructed. pCRP can accept heavy- and light-chain cDNAs cloned from combinatorial antibody libraries displayed on filamentous phages with the pCombIII system and is able to direct expression to soluble Fabs in which the carboxy-terminus of the heavy chain is fused to the amino-terminus of the mature PhoN nonspecific acid phosphatase of Providencia stuartii. Using the pCRP vector, we expressed two different human recombinant Fabs cloned from combinatorial libraries (one anti-tetenus toxoid and the other anti-HIV-1 gp120) fused with the acid phosphatase. In both cases chimeric antibodies were obtained which retained the antigen-binding ability and the enzymatic activity. Similar Fab-enzyme fusions can be successfully used, even unpurified, in enzyme immunoassays.
Lingua originaleEnglish
pagine (da-a)127-133
Numero di pagine7
RivistaNew Microbiologica
Volume18
Stato di pubblicazionePubblicato - 1995

Keywords

  • Acid Phosphatase
  • Antibodies, Monoclonal
  • Base Sequence
  • Gene Library
  • Genes, Bacterial
  • Genetic Vectors
  • HIV Envelope Protein gp120
  • HIV-1
  • Humans
  • Immunoglobulin Fab Fragments
  • Molecular Sequence Data
  • Providencia
  • Recombinant Fusion Proteins
  • Tetanus Toxoid

Fingerprint Entra nei temi di ricerca di 'Engineering human monoclonal antibody fragments: a recombinant enzyme-linked Fab'. Insieme formano una fingerprint unica.

Cita questo