Recently, we reported the short-term changes in the intestinal bacterial community of weaning mice induced by a diet high in unsaturated fat (1). Here, we investigate the effects of both saturated and unsaturated fat diets on caecal microbiota and physiological features of adult mice over a prolonged period of time. C57BL/6 mice were fed either a low-fat diet (LFD); a high-fat, soy oil-based diet (HFSD); or a high-fat, coconut oil-based diet (HFCD). Six mice from each group were killed after 2 (W2) and 8 weeks (W8) of dietary exposure. Daily weight gain and abdominal fat were determined. Samples of blood for metabolic status assessment, caecum tissue for histological evaluation and caecum content for microbiology analysis by DGGE (2) were collected. HFSD and HFCD mice showed a higher daily weight gain both at W2 and W8, and an increased abdominal fat as compared to LFD mice (P<0.05). Blood cholesterol was higher in HFSD (W2) and HFCD (W2 and W8) than in LFD mice (P<0.05). At W8 the histological analysis of the caecum evidenced that HFSD mice showed an increment of lesions of the mucosa (P≤0.01) and inflammatory cell infiltration (P≤0.05) compared to LFD. Visual inspection of the DGGE gel showed diet-dependent differences in band intensities: a band of reduced intensity in both HFSD and HFCD mice at W2 and W8 was identified as Lactobacillus gasseri, whereas two bands of increased intensity as compared to LFD mice were related to Clostridium populeti and Gordonibacter pamelaeae upon sequencing. DGGE data were confirmed by real-time PCR data. Cluster analysis of DGGE fingerprints revealed that HFSD and HFCD mice samples were distinctly grouped from the LFD control mice both at W2 and W8. These results corroborate previous findings on the impact of dietary fat on the gut microbial ecosystem and the physiology of the host. 1. Patrone, V., et al. (2012) Short-term modifications in the distal gut microbiota of weaning mice induced by a high-fat diet. Microbiology 158, 983-992 2. Walter, J., et al. (2000) Detection and identification of gastrointestinal Lactobacillus species by using denaturing gradient gel electrophoresis and species-specific PCR primers. Appl. Environ. Microbiol. 66, 297–303
Lingua originaleEnglish
Titolo della pubblicazione ospiteGut microbiota: friend or foe?
Numero di pagine1
Stato di pubblicazionePubblicato - 2012
Evento8th INRA-Rowett Symposium on Gut Microbiology - Clermont-Ferrand
Durata: 17 lug 201220 lug 2012


Convegno8th INRA-Rowett Symposium on Gut Microbiology


  • high-fat diets
  • microbiota
  • mouse


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