TY - JOUR
T1 - Effects of proton pump inhibitors on the gastric mucosa-associated microbiota in dyspeptic patients
AU - Sterbini, Francesco Paroni
AU - Palladini, Alessandra
AU - Masucci, Luca
AU - Cannistraci, Carlo Vittorio
AU - Pastorino, Roberta
AU - Ianiro, Gianluca
AU - Bugli, Francesca
AU - Martini, Cecilia
AU - Ricciardi, Walter
AU - Gasbarrini, Antonio
AU - Sanguinetti, Maurizio
AU - Cammarota, Giovanni
AU - Posteraro, Brunella
PY - 2016
Y1 - 2016
N2 - Besides being part of anti-Helicobacter pylori treatment regimens, proton pump inhibitors (PPIs) are increasingly being used to treat dyspepsia. However, little is known about the effects of PPIs on the human gastric microbiota, especially those related to H. pylori infection. The goal of this study was to characterize the stomach microbial communities in patients with dyspepsia and to investigate their relationships with PPI use and H. pylori status. Using 16S rRNA gene pyrosequencing, we analyzed the mucosa- associated microbial populations of 24 patients, of whom 12 were treated with the PPI omeprazole and 9 (5 treated and 4 untreated) were positive for H. pylori infection. The Proteobacteria, Firmicutes, Bacteroidetes, Fusobacteria, and Actinobacteria phyla accounted for 98% of all of the sequences, with Helicobacter, Streptococcus, and Prevotella ranking among the 10 most abundant genera. H. pylori infection or PPI treatment did not significantly influence gastric microbial species composition in dyspeptic patients. Principal-coordinate analysis of weighted UniFrac distances in these communities revealed clear but significant separation according to H. pylori status only. However, in PPI-treated patients, Firmicutes, particularly Streptococcaceae, were significantly increased in relative abundance compared to those in untreated patients. Consistently, Streptococcus was also found to significantly increase in relation to PPI treatment, and this increase seemed to occur independently of H. pylori infection. Our results suggest that Streptococcus may be a key indicator of PPI-induced gastric microbial composition changes in dyspeptic patients. Whether the gastric microbiota alteration contributes to dyspepsia needs further investigation.
AB - Besides being part of anti-Helicobacter pylori treatment regimens, proton pump inhibitors (PPIs) are increasingly being used to treat dyspepsia. However, little is known about the effects of PPIs on the human gastric microbiota, especially those related to H. pylori infection. The goal of this study was to characterize the stomach microbial communities in patients with dyspepsia and to investigate their relationships with PPI use and H. pylori status. Using 16S rRNA gene pyrosequencing, we analyzed the mucosa- associated microbial populations of 24 patients, of whom 12 were treated with the PPI omeprazole and 9 (5 treated and 4 untreated) were positive for H. pylori infection. The Proteobacteria, Firmicutes, Bacteroidetes, Fusobacteria, and Actinobacteria phyla accounted for 98% of all of the sequences, with Helicobacter, Streptococcus, and Prevotella ranking among the 10 most abundant genera. H. pylori infection or PPI treatment did not significantly influence gastric microbial species composition in dyspeptic patients. Principal-coordinate analysis of weighted UniFrac distances in these communities revealed clear but significant separation according to H. pylori status only. However, in PPI-treated patients, Firmicutes, particularly Streptococcaceae, were significantly increased in relative abundance compared to those in untreated patients. Consistently, Streptococcus was also found to significantly increase in relation to PPI treatment, and this increase seemed to occur independently of H. pylori infection. Our results suggest that Streptococcus may be a key indicator of PPI-induced gastric microbial composition changes in dyspeptic patients. Whether the gastric microbiota alteration contributes to dyspepsia needs further investigation.
KW - Applied Microbiology and Biotechnology
KW - Biotechnology
KW - Ecology
KW - Food Science
KW - Applied Microbiology and Biotechnology
KW - Biotechnology
KW - Ecology
KW - Food Science
UR - http://hdl.handle.net/10807/92110
UR - http://aem.asm.org/content/82/22/6633.full.pdf
U2 - 10.1128/AEM.01437-16
DO - 10.1128/AEM.01437-16
M3 - Article
SN - 0099-2240
VL - 82
SP - 6633
EP - 6644
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
ER -