Effects of palladium nanoparticles on the cytokine release from peripheral blood mononuclear cells of palladium-sensitized women

Ivo Iavicoli, M Reale, G Vianale, Lv Lotti, R Mariani Costantini, S Perconti, A Cristaudo, K Leopold, A Antonucci, L Di Giampaolo, M Di Gioacchino, P. Boscolo

Risultato della ricerca: Contributo in rivistaArticolo in rivista

26 Citazioni (Scopus)

Abstract

OBJECTIVE: To investigate the effects of palladium (Pd) nanoparticles on cytokine release from peripheral blood mononuclear cells (PBMCs) of control or Pd-sensitized nonatopic women. METHODS: TNF-alpha, IL-5, IL-10 and IFN-gamma release and/or expression from PBMCs incubated in presence of 5 to 10 nm Pd nanoparticles or Pd salt (potassium hexachloropalladate) were determined by enzyme-linked immunosorbent assay and reverse transcription-polymerase chain reaction analysis. Transmission electron microscopy was performed. RESULTS: In lipopolysaccharide-stimulated PBMCs from controls, Pd salt inhibited IFN-gamma and IL-10 release, whereas Pd nanoparticles enhanced IFN-gamma release and inhibited TNF-alpha secretion. In lipopolysaccharide-stimulated PBMCs from Pd-sensitized women showing high IFN-gamma release, Pd nanoparticles inhibited TNF-alpha release and Pd salt IL-10 release. TNF-alpha and IFN-gamma release and messenger RNA expression were correlated. Transmission electronmicroscopy demonstrated uptake of nanoparticles in the endocytic compartment and activation of autophagy. CONCLUSIONS: Palladium ions and nanoparticles exert different effects in vitro on the expression and release of cytokines.
Lingua originaleEnglish
pagine (da-a)1054-1060
Numero di pagine7
RivistaJournal of occupational and environmental medicine / American College of Occupational and Environmental Medicine
Volume53
DOI
Stato di pubblicazionePubblicato - 2011
Pubblicato esternamente

Keywords

  • Adult
  • Cells, Cultured
  • Cytokines
  • Dermatitis, Allergic Contact
  • Female
  • Gene Expression
  • Humans
  • Interferon-gamma
  • Interleukin-10
  • Interleukin-5
  • Leukocytes, Mononuclear
  • Middle Aged
  • Nanoparticles
  • Palladium
  • RNA, Messenger
  • Tumor Necrosis Factor-alpha
  • Young Adult

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