Effects of granulocyte-macrophage colony-stimulating factor and interleukin-3 on small cell lung cancer cells

Risultato della ricerca: Contributo in rivistaArticolo in rivista

Abstract

Nonhematopoietic malignant cells may express receptors for hematopoietic growth factors and respond to these peptides. The aim of the present study was to investigate whether small cell lung cancer (SCLC) cells may be stimulated to proliferate by hematopoietic growth factors such as granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-3 (IL-3), which are currently used in clinical trials in combination with cytotoxic chemotherapy. We studied two SCLC cell lines, H69 and N417. The effects of GM-CSF and IL-3 were evaluated by studying clonal growth, H-3-thymidine incorporation, BUIDR/DNA bivariate flow cytometry, c-myc and N-myc oncogene expression, and myeloid surface markers.Our experiments show that both GM-CSF and IL-3 can increase H-3-thymidine incorporation and cloning efficiency and reduce DNA synthesis time of H69 and, to a lesser extent, N417 cells, supporting the hypothesis that hematopoietic growth factors can stimulate the growth of some malignant nonhematopoietic cells in vitro. Further in vitro and in vivo studies are needed to determine whether clinical trials applying these factors for bone marrow recovery after chemotherapy of solid tumors may be hazardous by potentially stimulating growth of remaining tumor tissue.
Lingua originaleEnglish
pagine (da-a)283-288
Numero di pagine6
RivistaCancer Investigation
Volume12
DOI
Stato di pubblicazionePubblicato - 1994

Keywords

  • granulocyte-Macrophage Colony-Stimulating Factor / pharmacology

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