Effects of different amyloid β-protein analogues on synaptic function

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

48 Citazioni (Scopus)

Abstract

Perisynaptic accumulations of amyloid β-protein (Aβ) play a critical role in the synaptic dysfunction underlying the cognitive impairment observed in Alzheimer's disease. The methionine residue at position 35 (Met35) in Aβ is highly subject to oxidation in Alzheimer's disease brains. In hippocampal brain slices we found that long-term potentiation at CA3-CA1 synapses was significantly inhibited by wild type Aβ42 in which Met35 is reduced, but not by Aβ42 harboring Met35 sulfoxide. Similar differences were observed when basal synaptic transmission was investigated in autaptic hippocampal neurons. The significant decreases in excitatory postsynaptic current amplitude, vesicle release probability and miniature excitatory postsynaptic current frequency caused by 20-minute exposure to wild type Aβ42 were not observed after exposure to Aβ42 harboring Met35 sulfoxide. With longer (24-hour) Aβ treatments, this early impairment of the presynaptic terminal function extended to involve the postsynaptic side as well. The Met35 oxidation also affected Aβ42 negative impact on dendritic spine density and expression of pre- and postsynaptic proteins (synaptophysin and postsynaptic density protein-95). Our findings suggest that oxidation of Met35 is critical for molecular, structural, and functional determinants of Aβ42 synaptotoxicity.
Lingua originaleEnglish
pagine (da-a)1032-1044
Numero di pagine13
RivistaNeurobiology of Aging
Volume34
DOI
Stato di pubblicazionePubblicato - 2013

Keywords

  • Amyloid-b protein
  • Dentritic spines
  • Long-term potentiation
  • Synaptic transmission

Fingerprint

Entra nei temi di ricerca di 'Effects of different amyloid β-protein analogues on synaptic function'. Insieme formano una fingerprint unica.

Cita questo