Effect of prenatal steroidal inhibition of sPLA2 in a rat model of preterm lung

Daniele De Luca, Angelo Minucci, Krizia Pocino, Ettore Domenico Capoluongo, Ana Remesal, Laura San Feliciano, Maria Isidoro-Garcia, Josefina Casas, Gemma Fabrias, Dolores Ludena De La Cruz

Risultato della ricerca: Contributo in rivistaArticolo in rivista

4 Citazioni (Scopus)

Abstract

Introduction: Secretory phospholipase A2 regulates surfactant catabolism and inflammatory cascade. This enzyme is correlated with compliance, oxygenation and major outcomes in various forms of acute respiratory failure. Steroids inhibit secretory phospholipase A2 in cell culture and are widely used to boost surfactant production before preterm delivery. No data are available about the effect of antenatal steroids on secretory phospholipase A2 in the offspring: we aimed to study this effect in a rat model of preterm lung. Material and methods: Fifteen pregnant Wistar rats were randomized to receive betamethasone, dexamethasone or placebo at 20 and 21 days gestation. Newborn rats were supported for 8 h and then sacrificed: lung tissue was analysed for secretory phospholipase A2 expression and activity, inflammatory mediators and protein content. Lipidomics was analysed using liquid chromatography-mass spectrometry. Results: Secretory phospholipase A2 expression was significantly reduced by antenatal steroids (p < 0.001). Secretory phospholipase A2 activity, TNF alpha and lysophosphatidylethanolamine, a product of phospholipase reaction, were lowest in betamethasone-treated rats (p < 0.001). There was a strong correlation between secretory phospholipase A2 activity and lysophosphatidylethanolamine (r = 0.75; p = 0.001) and this remained significant after adjustment for total proteins or phospholipids. Conclusions: Antenatal steroids decrease secretory phospholipase A2 in rat model of preterm lung.
Lingua originaleEnglish
pagine (da-a)31-36
Numero di pagine6
RivistaPULMONARY PHARMACOLOGY &amp; THERAPEUTICS
Volume36
DOI
Stato di pubblicazionePubblicato - 2016

Keywords

  • Preterm neonate
  • Secretory phospholipase A2

Fingerprint

Entra nei temi di ricerca di 'Effect of prenatal steroidal inhibition of sPLA2 in a rat model of preterm lung'. Insieme formano una fingerprint unica.

Cita questo