TY - JOUR
T1 - Effect of activin-A on progesterone synthesis in human luteal cells
AU - Di Simone, Nicoletta
AU - Lanzone, Antonio
AU - Petraglia, Federica
AU - Ronsisvalle, E
AU - Caruso, Alessandro
AU - Mancuso, Salvatore
PY - 1994
Y1 - 1994
N2 - OBJECTIVE: To examine the effect of activin-A on basal and hCG-stimulated P production by human luteal cells.\r\n\r\nDESIGN: Mixed luteal cell cultures and distinct cultures of two luteal cell types: small and large luteal cells from early and midluteal phase.\r\n\r\nSETTING: Corpora lutea (CL) were obtained from the Obstetrics and Gynecology Department of the Catholic University, Rome, Italy.\r\n\r\nPATIENTS: Fifteen nonpregnant women between 30 and 45 years of age underwent surgery for nonendocrine gynecological diseases.\r\n\r\nINTERVENTIONS: Corpora lutea were obtained at the time of hysterectomy. The luteal cells were dispersed in Ham's F-12 medium containing collagenase at 37 degrees C in shaking water bath for 2 hours, filtered, centrifuged, and resuspended in fresh medium.\r\n\r\nMAIN OUTCOME MEASURES: The cells diluted to a final concentration of 60,000 to 100,000 cells/mL of medium. After 24 hours, the cells attached to the wells and were incubated with or without hCG and/or activin-A at different concentrations.\r\n\r\nRESULTS: Activin-A starting from 25 micrograms/L significantly decreased basal and hCG (250 mIU/mL [conversion to SI unit, 1.00])-induced P production by mixed luteal cells. The small luteal cells responded to hCG (250 mIU/mL), and the treatment with activin-A (from 25 to 100 micrograms/L) reduced their basal and hCG-induced P production. Activin-A addition did not change the amount of P release by large luteal cells at any concentration.\r\n\r\nCONCLUSIONS: These results imply that activin-A plays a role in the local regulation of human CL.
AB - OBJECTIVE: To examine the effect of activin-A on basal and hCG-stimulated P production by human luteal cells.\r\n\r\nDESIGN: Mixed luteal cell cultures and distinct cultures of two luteal cell types: small and large luteal cells from early and midluteal phase.\r\n\r\nSETTING: Corpora lutea (CL) were obtained from the Obstetrics and Gynecology Department of the Catholic University, Rome, Italy.\r\n\r\nPATIENTS: Fifteen nonpregnant women between 30 and 45 years of age underwent surgery for nonendocrine gynecological diseases.\r\n\r\nINTERVENTIONS: Corpora lutea were obtained at the time of hysterectomy. The luteal cells were dispersed in Ham's F-12 medium containing collagenase at 37 degrees C in shaking water bath for 2 hours, filtered, centrifuged, and resuspended in fresh medium.\r\n\r\nMAIN OUTCOME MEASURES: The cells diluted to a final concentration of 60,000 to 100,000 cells/mL of medium. After 24 hours, the cells attached to the wells and were incubated with or without hCG and/or activin-A at different concentrations.\r\n\r\nRESULTS: Activin-A starting from 25 micrograms/L significantly decreased basal and hCG (250 mIU/mL [conversion to SI unit, 1.00])-induced P production by mixed luteal cells. The small luteal cells responded to hCG (250 mIU/mL), and the treatment with activin-A (from 25 to 100 micrograms/L) reduced their basal and hCG-induced P production. Activin-A addition did not change the amount of P release by large luteal cells at any concentration.\r\n\r\nCONCLUSIONS: These results imply that activin-A plays a role in the local regulation of human CL.
KW - ACTIVIN-A
KW - PROGESTERONE
KW - ACTIVIN-A
KW - PROGESTERONE
UR - https://publicatt.unicatt.it/handle/10807/101885
UR - https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=0027987363&origin=inward
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0027987363&origin=inward
U2 - 10.1016/s0015-0282(16)57178-7
DO - 10.1016/s0015-0282(16)57178-7
M3 - Article
SN - 0015-0282
VL - 62
SP - 1157
EP - 1161
JO - Fertility and Sterility
JF - Fertility and Sterility
IS - 6
ER -