Abstract
The use of 2,2,2-trifluoroethanol-water mixtures for peptide separations
by capillary zone electrophoresis (CZE) displays some advantages over
aqueous solutions. First, the increase in viscosity reduces and
stabilizes the running current and facilitates heat dispersion, with a
consequent improvement in the number of theoretical plates. Second, the
decrease in the dielectric constant leads to a modification of the
dissociation constants of the ionizable groups. The consequence is a
change in selectivity that, for several favourable peptide pairs,
provides an increase in resolution. Third, the interaction of
trifluoroethanol with the peptide modifies the Stokes radius in a manner
strongly dependent on the peptide sequence. This can also be utilized
for an increase in CZE performance. Fourth, the structural properties of
2,2,2-trifluoroethanol are particularly useful for an improvement in the
separation of large apolar peptides. Finally, the use of
trifluoroethanol strongly stabilizes the capillary coating.
Lingua originale | English |
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pagine (da-a) | 271-281 |
Numero di pagine | 11 |
Rivista | JOURNAL OF CHROMATOGRAPHY A |
Volume | 735 |
DOI | |
Stato di pubblicazione | Pubblicato - 1996 |
Keywords
- CAPILLARY