Effect of 2,2,2-trifluoroethanol on capillary zone electrophoretic peptide separations

Massimo Castagnola, Loredana Cassiano, I Messana, M Paci, Diana Valeria Rossetti, Bruno Giardina

Risultato della ricerca: Contributo in rivistaArticolo in rivista

34 Citazioni (Scopus)


The use of 2,2,2-trifluoroethanol-water mixtures for peptide separations by capillary zone electrophoresis (CZE) displays some advantages over aqueous solutions. First, the increase in viscosity reduces and stabilizes the running current and facilitates heat dispersion, with a consequent improvement in the number of theoretical plates. Second, the decrease in the dielectric constant leads to a modification of the dissociation constants of the ionizable groups. The consequence is a change in selectivity that, for several favourable peptide pairs, provides an increase in resolution. Third, the interaction of trifluoroethanol with the peptide modifies the Stokes radius in a manner strongly dependent on the peptide sequence. This can also be utilized for an increase in CZE performance. Fourth, the structural properties of 2,2,2-trifluoroethanol are particularly useful for an improvement in the separation of large apolar peptides. Finally, the use of trifluoroethanol strongly stabilizes the capillary coating.
Lingua originaleEnglish
pagine (da-a)271-281
Numero di pagine11
RivistaJournal of Chromatography A
Stato di pubblicazionePubblicato - 1996




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