EFFECT OF 2-METHOXY-5-NITROTROPONE ON OXYGEN-AFFINITY OF HUMAN ERYTHROCYTES AND HEMOGLOBIN

D Currell; E Benitez; C Ioppolo; Bruno Giardina; S Condo; F Martini; E. Antonini

doi:10.1111/j.1432-1033.1978.tb20964.x

EFFECT OF 2-METHOXY-5-NITROTROPONE ON OXYGEN-AFFINITY OF HUMAN ERYTHROCYTES AND HEMOGLOBIN

D Currell, E Benitez, C Ioppolo, Bruno Giardina, S Condo, F Martini, E. Antonini

Risultato della ricerca: Contributo in rivista › Articolo in rivista

3 Citazioni (Scopus)

Abstract

Human hemoglobin reacted with 2-methoxy-5-nitrotropone at pH 7.4 undergoes modification of the four N-terminal amino groups. The modified protein shows increased oxygen affinity with complete abolition of the effect of D-glycerate 2,3-bisphosphate. The Bohr effect is abolished in the acid range and drastically reduced at alkaline pH values. Changes in the Kinetics of ligand reactions parallel the oxygen equilibrium results. Cooperative effects are still present. Human erythrocytes treated with 2-methoxy-5-nitrotropone show increased oxygen affinity and some decrease in methemoglobin reductase efficiency but no change in resistance to hemolysis

Lingua originale	English
pagine (da-a)	285-289
Numero di pagine	5
Rivista	European Journal of Biochemistry
Volume	91
DOI	https://doi.org/10.1111/j.1432-1033.1978.tb20964.x
Stato di pubblicazione	Pubblicato - 1978
Pubblicato esternamente	Sì

Keywords

HEMOGLOBIN
HUMAN HERYTHROCYTES

Accesso al documento

10.1111/j.1432-1033.1978.tb20964.x

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title = "EFFECT OF 2-METHOXY-5-NITROTROPONE ON OXYGEN-AFFINITY OF HUMAN ERYTHROCYTES AND HEMOGLOBIN",

abstract = "Human hemoglobin reacted with 2-methoxy-5-nitrotropone at pH 7.4 undergoes modification of the four N-terminal amino groups. The modified protein shows increased oxygen affinity with complete abolition of the effect of D-glycerate 2,3-bisphosphate. The Bohr effect is abolished in the acid range and drastically reduced at alkaline pH values. Changes in the Kinetics of ligand reactions parallel the oxygen equilibrium results. Cooperative effects are still present. Human erythrocytes treated with 2-methoxy-5-nitrotropone show increased oxygen affinity and some decrease in methemoglobin reductase efficiency but no change in resistance to hemolysis",

keywords = "HEMOGLOBIN, HUMAN HERYTHROCYTES, HEMOGLOBIN, HUMAN HERYTHROCYTES",

author = "D Currell and E Benitez and C Ioppolo and Bruno Giardina and S Condo and F Martini and E. Antonini",

year = "1978",

doi = "10.1111/j.1432-1033.1978.tb20964.x",

language = "English",

volume = "91",

pages = "285--289",

journal = "European Journal of Biochemistry",

issn = "0014-2956",

publisher = "Blackwell Science Limited:PO Box 88, Oxford OX2 0NE United Kingdom:011 44 1865 776868, 011 44 1865 206038, EMAIL: [email protected], INTERNET: http://www.blackwell-science.com, Fax: 011 44 1865 721205",

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TY - JOUR

T1 - EFFECT OF 2-METHOXY-5-NITROTROPONE ON OXYGEN-AFFINITY OF HUMAN ERYTHROCYTES AND HEMOGLOBIN

AU - Currell, D

AU - Benitez, E

AU - Ioppolo, C

AU - Giardina, Bruno

AU - Condo, S

AU - Martini, F

AU - Antonini, E.

PY - 1978

Y1 - 1978

N2 - Human hemoglobin reacted with 2-methoxy-5-nitrotropone at pH 7.4 undergoes modification of the four N-terminal amino groups. The modified protein shows increased oxygen affinity with complete abolition of the effect of D-glycerate 2,3-bisphosphate. The Bohr effect is abolished in the acid range and drastically reduced at alkaline pH values. Changes in the Kinetics of ligand reactions parallel the oxygen equilibrium results. Cooperative effects are still present. Human erythrocytes treated with 2-methoxy-5-nitrotropone show increased oxygen affinity and some decrease in methemoglobin reductase efficiency but no change in resistance to hemolysis

AB - Human hemoglobin reacted with 2-methoxy-5-nitrotropone at pH 7.4 undergoes modification of the four N-terminal amino groups. The modified protein shows increased oxygen affinity with complete abolition of the effect of D-glycerate 2,3-bisphosphate. The Bohr effect is abolished in the acid range and drastically reduced at alkaline pH values. Changes in the Kinetics of ligand reactions parallel the oxygen equilibrium results. Cooperative effects are still present. Human erythrocytes treated with 2-methoxy-5-nitrotropone show increased oxygen affinity and some decrease in methemoglobin reductase efficiency but no change in resistance to hemolysis

KW - HEMOGLOBIN

KW - HUMAN HERYTHROCYTES

KW - HEMOGLOBIN

KW - HUMAN HERYTHROCYTES

UR - http://hdl.handle.net/10807/6885

U2 - 10.1111/j.1432-1033.1978.tb20964.x

DO - 10.1111/j.1432-1033.1978.tb20964.x

M3 - Article

SN - 0014-2956

VL - 91

SP - 285

EP - 289

JO - European Journal of Biochemistry

JF - European Journal of Biochemistry

ER -

EFFECT OF 2-METHOXY-5-NITROTROPONE ON OXYGEN-AFFINITY OF HUMAN ERYTHROCYTES AND HEMOGLOBIN

Abstract

Keywords

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