EFFECT OF 2-METHOXY-5-NITROTROPONE ON OXYGEN-AFFINITY OF HUMAN ERYTHROCYTES AND HEMOGLOBIN

Bruno Giardina, D Currell, E Benitez, C Ioppolo, S Condo, F Martini, E. Antonini

Risultato della ricerca: Contributo in rivistaArticolo in rivista

3 Citazioni (Scopus)

Abstract

Human hemoglobin reacted with 2-methoxy-5-nitrotropone at pH 7.4 undergoes modification of the four N-terminal amino groups. The modified protein shows increased oxygen affinity with complete abolition of the effect of D-glycerate 2,3-bisphosphate. The Bohr effect is abolished in the acid range and drastically reduced at alkaline pH values. Changes in the Kinetics of ligand reactions parallel the oxygen equilibrium results. Cooperative effects are still present. Human erythrocytes treated with 2-methoxy-5-nitrotropone show increased oxygen affinity and some decrease in methemoglobin reductase efficiency but no change in resistance to hemolysis
Lingua originaleEnglish
pagine (da-a)285-289
Numero di pagine5
RivistaEuropean Journal of Biochemistry
Volume91
DOI
Stato di pubblicazionePubblicato - 1978

Keywords

  • HEMOGLOBIN
  • HUMAN HERYTHROCYTES

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