Early activation of fibrogenesis in transplanted kidneys: a study on serial renal biopsies.

Giovanni Gambaro, Dorella Del Prete, Monica Ceol, Franca Anglani, Daniela Vianello, Emilia Tiralongo, Marialuisa Valente, Romina Graziotto, Luciana Bonfante, Giuseppe Scaparrotta, Lucrezia Furian, Paolo Rigotti, Angela D'Angelo

Risultato della ricerca: Contributo in rivistaArticolo in rivista

10 Citazioni (Scopus)

Abstract

BACKGROUND/AIMS: In kidney transplants, the renin-angiotensin system (RAS) is involved in systemic and local changes that may induce fibrosis. Our aim was to use gene expression and immunohistochemical analysis to investigate the RAS and several factors involved in the fibrogenic cascade in allograft biopsies. METHODS: We considered 43 donor biopsies (T0), 18 biopsies obtained for diagnostic purposes (Td) and 24 protocol biopsies (Tp) taken 2 months after transplantation in patients with stable renal function. Morphometric alpha SMA and TGF beta 1 analysis, and Masson's Trichrome staining were performed. mRNA levels of angiotensinogen, renin, ACE, AT1-R, AT2-R, TGF beta 1, BMP-7, Coll III, fibronectin and alpha SMA were analyzed by real-time RT/PCR. MDRD a year after the transplant was also considered. RESULTS: Significantly higher levels of AT1-R and alpha SMA transcripts were found in Tp than in T0. Regression analysis showed significant TGF beta 1-independent positive correlations between RAS and matrix components in T0 and Tp, but more evident in Tp, where a positive correlation between TGF beta 1 and Masson's Trichrome stained areas was also seen. CONCLUSION: Our results suggest that RAS and TGF beta 1-related fibrogenic loops are activated as early as 2 months after kidney transplantation.
Lingua originaleEnglish
pagine (da-a)141-145
Numero di pagine5
RivistaExperimental and Molecular Pathology
Volume87
Stato di pubblicazionePubblicato - 2009

Keywords

  • fibrogenesis
  • kidney
  • transplantation

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