Abstract
A quick, sensitive and easily automatizable method for PCR amplification of genomic DNA eluted from dried blood spots is described. DNA is eluted from a 3-mm spot routinely used for neonatal screening of inherited diseases either by boiling or by sonication. A preliminary and brief spot-autoclaving step is mandatory to ensure optimal and reproducible PCR amplifications. Only 1% of the eluted DNA is required for PCR analysis allowing the execution of multiple genetic tests on the same blood spot. The method has been successfully applied to the detection of a known phenylketonuria-causing mutation and will facilitate the analysis of the genetic repository provided by Guthrie's cards stored in neonatal screening laboratories.
Lingua originale | English |
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pagine (da-a) | 735-737 |
Numero di pagine | 3 |
Rivista | BioTechniques |
Volume | 13 |
Stato di pubblicazione | Pubblicato - 1992 |
Keywords
- Base Sequence
- DNA
- DNA Probes
- Female
- Humans
- Male
- Molecular Sequence Data
- Polymerase Chain Reaction