DNA elution and amplification by polymerase chain reaction from dried blood spots

Alfredo Pontecorvi, C. Carducci, L. Ellul, I. Antonozzi

Risultato della ricerca: Contributo in rivistaArticolo in rivista

30 Citazioni (Scopus)

Abstract

A quick, sensitive and easily automatizable method for PCR amplification of genomic DNA eluted from dried blood spots is described. DNA is eluted from a 3-mm spot routinely used for neonatal screening of inherited diseases either by boiling or by sonication. A preliminary and brief spot-autoclaving step is mandatory to ensure optimal and reproducible PCR amplifications. Only 1% of the eluted DNA is required for PCR analysis allowing the execution of multiple genetic tests on the same blood spot. The method has been successfully applied to the detection of a known phenylketonuria-causing mutation and will facilitate the analysis of the genetic repository provided by Guthrie's cards stored in neonatal screening laboratories.
Lingua originaleEnglish
pagine (da-a)735-737
Numero di pagine3
RivistaBioTechniques
Volume13
Stato di pubblicazionePubblicato - 1992

Keywords

  • Base Sequence
  • DNA
  • DNA Probes
  • Female
  • Humans
  • Male
  • Molecular Sequence Data
  • Polymerase Chain Reaction

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