TY - JOUR
T1 - Disruption of the gene encoding glutamate dehydrogenase affects growth, amino acids catabolism and survival of Lactobacillus plantarum UC1001
AU - Siragusa, Sonya
AU - Fontana, Cecilia Alejandra
AU - Cappa, Fabrizio
AU - Caputo, Leonardo
AU - Cocconcelli, Pier Sandro
AU - Gobbetti, Marco
AU - De Angelis, Maria
PY - 2011
Y1 - 2011
N2 - The role played by glutamate dehydrogenase (GDH) in Lactobacillus plantarum was investigated by
preparing a GDH-deficient mutant UC1001G. The growth rate and acidification displayed by this mutant
were lower than those of the wild-type UC1001. Compared with UC1001G, UC1001 increased the
synthesis of proteins that confer a competitive advantage of cell resistance. When both strains were used
as adjunct starter for cheese-making, the highest cell survival was found for UC1001. Cheeses made with
the adjunct of UC1001 showed the lowest level of free amino acids and the highest content of volatile
organic compounds. Both strains produced high level of g-aminobutyric acid in cheese and under
simulated gastrointestinal conditions. The findings of this study provide an evidence of the important
role of GDH on growth and survival of Lb. plantarum under different environmental conditions that
influences some important functional features of the strain.
AB - The role played by glutamate dehydrogenase (GDH) in Lactobacillus plantarum was investigated by
preparing a GDH-deficient mutant UC1001G. The growth rate and acidification displayed by this mutant
were lower than those of the wild-type UC1001. Compared with UC1001G, UC1001 increased the
synthesis of proteins that confer a competitive advantage of cell resistance. When both strains were used
as adjunct starter for cheese-making, the highest cell survival was found for UC1001. Cheeses made with
the adjunct of UC1001 showed the lowest level of free amino acids and the highest content of volatile
organic compounds. Both strains produced high level of g-aminobutyric acid in cheese and under
simulated gastrointestinal conditions. The findings of this study provide an evidence of the important
role of GDH on growth and survival of Lb. plantarum under different environmental conditions that
influences some important functional features of the strain.
KW - Lactobacillus plantarum
KW - Lactobacillus plantarum
UR - http://hdl.handle.net/10807/7837
M3 - Article
SN - 0958-6946
SP - 59
EP - 68
JO - International Dairy Journal
JF - International Dairy Journal
ER -