TY - JOUR
T1 - Discrimination of extra-virgin-olive oils from different cultivars and geographical origins by untargeted metabolomics
AU - Ghisoni, Silvia
AU - Lucini, Luigi
AU - Angilletta, Federica
AU - Rocchetti, Gabriele
AU - Farinelli, Daniela
AU - Tombesi, Sergio
AU - Trevisan, Marco
PY - 2018
Y1 - 2018
N2 - In this preliminary study, ultra-high-pressure liquid chromatography (UHPLC) coupled to quadrupole-time-of-flight mass spectrometry (QTOF) metabolomics followed by multivariate statistics was applied to discriminate nine extra-virgin olive oil (EVOO) cultivars according to their phenolic and sterolic fingerprints. The same approach was then used to discriminate EVOO samples from different geographical origins, namely six blends representative of the main growing regions in Italy (i.e., Sicily, Puglia, Umbria, Liguria, Lombardy and Tuscany).
This approach allowed to putatively identify >1000 compounds, considering both polyphenols and sterols. The unsupervised hierarchical cluster analysis (HCA) and the orthogonal projections to latent structures discriminant analysis (OPLS-DA) discriminated EVOO samples according to both cultivar and geographical origin. In particular, flavonoids (i.e., anthocyanins and flavonols), hydroxycinnamic acids and cholesterol derivatives were found to be the most representative classes of compounds discriminating EVOO samples according to the two parameters (cultivar or geographical origin) selected. However, the following Venn analysis allowed to point out the discriminant markers being exclusive for cultivar or origin discrimination. In this regard, only the 9.6% of phenolics and 13.6% of sterols were in common, thus indicating that several of these discriminant compounds were exclusive of a single condition. Indeed, considering that most of the commercial EVOOs are blended, the contribution of both the cultivars used and the geographical origin must be taken into account.
AB - In this preliminary study, ultra-high-pressure liquid chromatography (UHPLC) coupled to quadrupole-time-of-flight mass spectrometry (QTOF) metabolomics followed by multivariate statistics was applied to discriminate nine extra-virgin olive oil (EVOO) cultivars according to their phenolic and sterolic fingerprints. The same approach was then used to discriminate EVOO samples from different geographical origins, namely six blends representative of the main growing regions in Italy (i.e., Sicily, Puglia, Umbria, Liguria, Lombardy and Tuscany).
This approach allowed to putatively identify >1000 compounds, considering both polyphenols and sterols. The unsupervised hierarchical cluster analysis (HCA) and the orthogonal projections to latent structures discriminant analysis (OPLS-DA) discriminated EVOO samples according to both cultivar and geographical origin. In particular, flavonoids (i.e., anthocyanins and flavonols), hydroxycinnamic acids and cholesterol derivatives were found to be the most representative classes of compounds discriminating EVOO samples according to the two parameters (cultivar or geographical origin) selected. However, the following Venn analysis allowed to point out the discriminant markers being exclusive for cultivar or origin discrimination. In this regard, only the 9.6% of phenolics and 13.6% of sterols were in common, thus indicating that several of these discriminant compounds were exclusive of a single condition. Indeed, considering that most of the commercial EVOOs are blended, the contribution of both the cultivars used and the geographical origin must be taken into account.
KW - Extra-Virgin Olive Oil
KW - Food metabolomics
KW - Polyphenols
KW - Sterols
KW - Traceability
KW - Extra-Virgin Olive Oil
KW - Food metabolomics
KW - Polyphenols
KW - Sterols
KW - Traceability
UR - http://hdl.handle.net/10807/128987
U2 - 10.1016/j.foodres.2018.12.052
DO - 10.1016/j.foodres.2018.12.052
M3 - Article
SN - 0963-9969
SP - N/A-N/A
JO - Food Research International
JF - Food Research International
ER -