Direct testing for kpc-mediated carbapenem resistance from blood samples using a t2 magnetic resonance based assay

Giulia De Angelis; R. Paggi; T. J. Lowery; J. L. Snyder; G. Menchinelli; Maurizio Sanguinetti; Brunella Posteraro; A. Mencacci

doi:10.3390/antibiotics10080950

Direct testing for kpc-mediated carbapenem resistance from blood samples using a t2 magnetic resonance based assay

Giulia De Angelis, R. Paggi, T. J. Lowery, J. L. Snyder, G. Menchinelli, Maurizio Sanguinetti^*, Brunella Posteraro, A. Mencacci

^*Autore corrispondente per questo lavoro

Risultato della ricerca: Contributo in rivista › Articolo

Abstract

Molecular-based carbapenem resistance testing in Gram-negative bacterial bloodstream infections (BSIs) is currently limited because of the reliance on positive blood culture (BC) samples. The T2Resistance™ panel may now allow the detection of carbapenemase-and other β-lactamase encoding genes directly from blood samples. We detected carbapenem resistance genes in 11 (84.6%) of 13 samples from patients with BC-documented BSIs (10 caused by KPC-producing Klebsiella pneumoniae and 1 caused by VIM/CMY-producing Citrobacter freundii). Two samples that tested negative for carbapenem resistance genes were from patients with BC-documented BSIs caused by KPC-producing K. pneumoniae who were receiving effective antibiotic therapy. In conclusion, our findings suggest that the T2Resistance™ panel can be a reliable tool for diagnosing carbapenem-resistant Gram-negative bacterial BSIs.

Lingua originale	Inglese
pagine (da-a)	950-N/A
Rivista	Antibiotics
Volume	10
Numero di pubblicazione	8
DOI	https://doi.org/10.3390/antibiotics10080950
Stato di pubblicazione	Pubblicato - 2021

All Science Journal Classification (ASJC) codes

Microbiologia
Biochimica
Farmacologia, Tossicologia e Farmaceutica Generali
Microbiologia (medica)
Malattie Infettive
Farmacologia (medica)

Keywords

Antimicrobial resistance
Blood sample
Bloodstream infection
Direct detection
KPC carbapenemase
Magnetic resonance
T2Resistance panel

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10.3390/antibiotics10080950

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title = "Direct testing for kpc-mediated carbapenem resistance from blood samples using a t2 magnetic resonance based assay",

abstract = "Molecular-based carbapenem resistance testing in Gram-negative bacterial bloodstream infections (BSIs) is currently limited because of the reliance on positive blood culture (BC) samples. The T2Resistance{\texttrademark} panel may now allow the detection of carbapenemase-and other β-lactamase encoding genes directly from blood samples. We detected carbapenem resistance genes in 11 (84.6%) of 13 samples from patients with BC-documented BSIs (10 caused by KPC-producing Klebsiella pneumoniae and 1 caused by VIM/CMY-producing Citrobacter freundii). Two samples that tested negative for carbapenem resistance genes were from patients with BC-documented BSIs caused by KPC-producing K. pneumoniae who were receiving effective antibiotic therapy. In conclusion, our findings suggest that the T2Resistance{\texttrademark} panel can be a reliable tool for diagnosing carbapenem-resistant Gram-negative bacterial BSIs.",

keywords = "Antimicrobial resistance, Blood sample, Bloodstream infection, Direct detection, KPC carbapenemase, Magnetic resonance, T2Resistance panel, Antimicrobial resistance, Blood sample, Bloodstream infection, Direct detection, KPC carbapenemase, Magnetic resonance, T2Resistance panel",

author = "{De Angelis}, Giulia and R. Paggi and Lowery, {T. J.} and Snyder, {J. L.} and G. Menchinelli and Maurizio Sanguinetti and Brunella Posteraro and A. Mencacci",

year = "2021",

doi = "10.3390/antibiotics10080950",

language = "English",

volume = "10",

pages = "950--N/A",

journal = "Antibiotics",

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T1 - Direct testing for kpc-mediated carbapenem resistance from blood samples using a t2 magnetic resonance based assay

AU - De Angelis, Giulia

AU - Paggi, R.

AU - Lowery, T. J.

AU - Snyder, J. L.

AU - Menchinelli, G.

AU - Sanguinetti, Maurizio

AU - Posteraro, Brunella

AU - Mencacci, A.

PY - 2021

Y1 - 2021

N2 - Molecular-based carbapenem resistance testing in Gram-negative bacterial bloodstream infections (BSIs) is currently limited because of the reliance on positive blood culture (BC) samples. The T2Resistance™ panel may now allow the detection of carbapenemase-and other β-lactamase encoding genes directly from blood samples. We detected carbapenem resistance genes in 11 (84.6%) of 13 samples from patients with BC-documented BSIs (10 caused by KPC-producing Klebsiella pneumoniae and 1 caused by VIM/CMY-producing Citrobacter freundii). Two samples that tested negative for carbapenem resistance genes were from patients with BC-documented BSIs caused by KPC-producing K. pneumoniae who were receiving effective antibiotic therapy. In conclusion, our findings suggest that the T2Resistance™ panel can be a reliable tool for diagnosing carbapenem-resistant Gram-negative bacterial BSIs.

AB - Molecular-based carbapenem resistance testing in Gram-negative bacterial bloodstream infections (BSIs) is currently limited because of the reliance on positive blood culture (BC) samples. The T2Resistance™ panel may now allow the detection of carbapenemase-and other β-lactamase encoding genes directly from blood samples. We detected carbapenem resistance genes in 11 (84.6%) of 13 samples from patients with BC-documented BSIs (10 caused by KPC-producing Klebsiella pneumoniae and 1 caused by VIM/CMY-producing Citrobacter freundii). Two samples that tested negative for carbapenem resistance genes were from patients with BC-documented BSIs caused by KPC-producing K. pneumoniae who were receiving effective antibiotic therapy. In conclusion, our findings suggest that the T2Resistance™ panel can be a reliable tool for diagnosing carbapenem-resistant Gram-negative bacterial BSIs.

KW - Antimicrobial resistance

KW - Blood sample

KW - Bloodstream infection

KW - Direct detection

KW - KPC carbapenemase

KW - Magnetic resonance

KW - T2Resistance panel

KW - Antimicrobial resistance

KW - Blood sample

KW - Bloodstream infection

KW - Direct detection

KW - KPC carbapenemase

KW - Magnetic resonance

KW - T2Resistance panel

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Direct testing for kpc-mediated carbapenem resistance from blood samples using a t2 magnetic resonance based assay

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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