Direct testing for kpc-mediated carbapenem resistance from blood samples using a t2 magnetic resonance based assay

Giulia De Angelis; Riccardo Paggi; Raffaela Paggi; Thomas J. Lowery; Jessica L. Snyder; Giulia Menchinelli; Maurizio Sanguinetti; Brunella Posteraro; Antonella Mencacci

doi:10.3390/antibiotics10080950

Direct testing for kpc-mediated carbapenem resistance from blood samples using a t2 magnetic resonance based assay

Giulia De Angelis, Riccardo Paggi, Raffaela Paggi, Thomas J. Lowery, Jessica L. Snyder, Giulia Menchinelli, Maurizio Sanguinetti, Brunella Posteraro, Antonella Mencacci

University of Perugia

Risultato della ricerca: Contributo in rivista › Articolo in rivista

Abstract

Molecular-based carbapenem resistance testing in Gram-negative bacterial bloodstream infections (BSIs) is currently limited because of the reliance on positive blood culture (BC) samples. The T2Resistance™ panel may now allow the detection of carbapenemase-and other β-lactamase encoding genes directly from blood samples. We detected carbapenem resistance genes in 11 (84.6%) of 13 samples from patients with BC-documented BSIs (10 caused by KPC-producing Klebsiella pneumoniae and 1 caused by VIM/CMY-producing Citrobacter freundii). Two samples that tested negative for carbapenem resistance genes were from patients with BC-documented BSIs caused by KPC-producing K. pneumoniae who were receiving effective antibiotic therapy. In conclusion, our findings suggest that the T2Resistance™ panel can be a reliable tool for diagnosing carbapenem-resistant Gram-negative bacterial BSIs.

Lingua originale	English
pagine (da-a)	950-N/A
Rivista	Antibiotics
Volume	10
DOI	https://doi.org/10.3390/antibiotics10080950
Stato di pubblicazione	Pubblicato - 2021

Keywords

Antimicrobial resistance
Blood sample
Bloodstream infection
Direct detection
KPC carbapenemase
Magnetic resonance
T2Resistance panel

Accesso al documento

10.3390/antibiotics10080950

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title = "Direct testing for kpc-mediated carbapenem resistance from blood samples using a t2 magnetic resonance based assay",

abstract = "Molecular-based carbapenem resistance testing in Gram-negative bacterial bloodstream infections (BSIs) is currently limited because of the reliance on positive blood culture (BC) samples. The T2Resistance{\texttrademark} panel may now allow the detection of carbapenemase-and other β-lactamase encoding genes directly from blood samples. We detected carbapenem resistance genes in 11 (84.6%) of 13 samples from patients with BC-documented BSIs (10 caused by KPC-producing Klebsiella pneumoniae and 1 caused by VIM/CMY-producing Citrobacter freundii). Two samples that tested negative for carbapenem resistance genes were from patients with BC-documented BSIs caused by KPC-producing K. pneumoniae who were receiving effective antibiotic therapy. In conclusion, our findings suggest that the T2Resistance{\texttrademark} panel can be a reliable tool for diagnosing carbapenem-resistant Gram-negative bacterial BSIs.",

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author = "{De Angelis}, Giulia and Riccardo Paggi and Raffaela Paggi and Lowery, {Thomas J.} and Snyder, {Jessica L.} and Giulia Menchinelli and Maurizio Sanguinetti and Brunella Posteraro and Antonella Mencacci",

year = "2021",

doi = "10.3390/antibiotics10080950",

language = "English",

volume = "10",

pages = "950--N/A",

journal = "Antibiotics",

issn = "2079-6382",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

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TY - JOUR

T1 - Direct testing for kpc-mediated carbapenem resistance from blood samples using a t2 magnetic resonance based assay

AU - De Angelis, Giulia

AU - Paggi, Riccardo

AU - Paggi, Raffaela

AU - Lowery, Thomas J.

AU - Snyder, Jessica L.

AU - Menchinelli, Giulia

AU - Sanguinetti, Maurizio

AU - Posteraro, Brunella

AU - Mencacci, Antonella

PY - 2021

Y1 - 2021

N2 - Molecular-based carbapenem resistance testing in Gram-negative bacterial bloodstream infections (BSIs) is currently limited because of the reliance on positive blood culture (BC) samples. The T2Resistance™ panel may now allow the detection of carbapenemase-and other β-lactamase encoding genes directly from blood samples. We detected carbapenem resistance genes in 11 (84.6%) of 13 samples from patients with BC-documented BSIs (10 caused by KPC-producing Klebsiella pneumoniae and 1 caused by VIM/CMY-producing Citrobacter freundii). Two samples that tested negative for carbapenem resistance genes were from patients with BC-documented BSIs caused by KPC-producing K. pneumoniae who were receiving effective antibiotic therapy. In conclusion, our findings suggest that the T2Resistance™ panel can be a reliable tool for diagnosing carbapenem-resistant Gram-negative bacterial BSIs.

AB - Molecular-based carbapenem resistance testing in Gram-negative bacterial bloodstream infections (BSIs) is currently limited because of the reliance on positive blood culture (BC) samples. The T2Resistance™ panel may now allow the detection of carbapenemase-and other β-lactamase encoding genes directly from blood samples. We detected carbapenem resistance genes in 11 (84.6%) of 13 samples from patients with BC-documented BSIs (10 caused by KPC-producing Klebsiella pneumoniae and 1 caused by VIM/CMY-producing Citrobacter freundii). Two samples that tested negative for carbapenem resistance genes were from patients with BC-documented BSIs caused by KPC-producing K. pneumoniae who were receiving effective antibiotic therapy. In conclusion, our findings suggest that the T2Resistance™ panel can be a reliable tool for diagnosing carbapenem-resistant Gram-negative bacterial BSIs.

KW - Antimicrobial resistance

KW - Blood sample

KW - Bloodstream infection

KW - Direct detection

KW - KPC carbapenemase

KW - Magnetic resonance

KW - T2Resistance panel

KW - Antimicrobial resistance

KW - Blood sample

KW - Bloodstream infection

KW - Direct detection

KW - KPC carbapenemase

KW - Magnetic resonance

KW - T2Resistance panel

UR - http://hdl.handle.net/10807/198167

U2 - 10.3390/antibiotics10080950

DO - 10.3390/antibiotics10080950

M3 - Article

SN - 2079-6382

VL - 10

SP - 950-N/A

JO - Antibiotics

JF - Antibiotics

ER -

Direct testing for kpc-mediated carbapenem resistance from blood samples using a t2 magnetic resonance based assay

Abstract

Keywords

Accesso al documento

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