Differential methylation pattern of the X-linked lymphoproliferative (XLP) disease gene SH2D1A correlates with the cell lineage-specific transcription

Ornella Parolini, Andreas Weinhäusel, Birgit Kagerbauer, Joachim Sassmann, Wolfgang Holter, Helmut Gadner, Oskar A. Haas, Walter Knapp

Risultato della ricerca: Contributo in rivistaArticolo in rivista

9 Citazioni (Scopus)

Abstract

SH2D1A, the X-linked lymphoproliferative disease (XLP) gene, encodes a cytoplasmic protein that plays an essential role in controlling Epstein-Barr virus infection. It is expressed in T and NK cells, but not in B cells or in granulocytes. The promoter, the regulatory regions, as well as the mechanisms controlling its tissue-specific expression, are still unknown. We tested the hypothesis that DNA methylation might contribute to tissue-specific SH2D1A gene expression and analyzed the methylation status of 2,300 bp upstream of the ATG starting codon, the coding region and part of intron 1. By bisulfite sequencing and methylation-sensitive restriction enzyme digestion, we show that a differential methylation pattern of CpG-rich regions in the 5' region and the adjacent exon 1 of the SH2D1A gene indeed correlates with the tissue-specific gene transcription.
Lingua originaleEnglish
pagine (da-a)116-121
Numero di pagine6
RivistaImmunogenetics
Volume55
DOI
Stato di pubblicazionePubblicato - 2003

Keywords

  • Animals
  • Binding Sites
  • Carrier Proteins
  • Computational Biology
  • CpG Islands
  • DNA Methylation
  • Gene Expression Regulation
  • Gene Silencing
  • Humans
  • Intracellular Signaling Peptides and Proteins
  • Lymphoproliferative Disorders
  • Sequence Analysis, DNA

Fingerprint Entra nei temi di ricerca di 'Differential methylation pattern of the X-linked lymphoproliferative (XLP) disease gene SH2D1A correlates with the cell lineage-specific transcription'. Insieme formano una fingerprint unica.

Cita questo